Description
Efficient Weak Anion Exchange for SMART™ Chromatography
ZetaCell DEAE from emp BIOTECH is a large-bead weak anion exchange resin designed specifically for SMART™ ion exchange chromatography. It features highly cross-linked agarose particles functionalized with a diethylaminoethyl (DEAE) ligand, ideal for the purification of negatively charged biomolecules under mild conditions.
Engineered for high-throughput purification directly from unclarified feed streams, ZetaCell DEAE supports predictable, linear scale-up and robust processing of mammalian cells, bacteria, yeast, and other bioreactor-derived materials. The 250 µm bead diameter ensures optimal flow properties and minimal risk of clogging, even in cell-containing samples.
Performance Highlights
- ≥ 80% particles in the 140–360 µm range
- Linear velocity ≥ 2000 cm/h
- Operates efficiently at up to 3 bar
- Ion exchange capacity: 0.08–0.16 mmol Cl⁻/mL
- Broad pH stability: 2–14 (short term), 3–12 (long term)
- Optimized for direct processing of raw feeds in SMART™ columns
Specifications
- Matrix: Highly cross-linked agarose
- Ligand: Diethylaminoethyl (DEAE), weak anion exchanger
- Bead Diameter: Average 250 µm
- Storage Buffer: 20% ethanol
Applications
- Weak anion exchange (AEX) chromatography
- Purification of negatively charged proteins and biomolecules
- Capture from clarified and unclarified feed streams
- Continuous bioprocessing and SMART™ workflows
Why Choose ZetaCell DEAE?
ZetaCell DEAE is tailored for SMART™ chromatography, offering enhanced process efficiency and reproducibility in cell-containing feeds. Its bead geometry and ligand accessibility enable high flow rates and low backpressure, supporting seamless scale-up from development to manufacturing. An ideal choice for gentle AEX separation in complex bioprocessing environments.
