Description
High-Throughput Anion Exchange for Clarified or Cell-Containing Feeds
ZetaCell Q from emp BIOTECH is a strong anion exchange resin designed for efficient purification of negatively charged biomolecules, including proteins, oligonucleotides, and nucleic acid complexes. Built for SMART™ Chromatography, it enables linear scalability from research to process scale with high flow rates and minimal backpressure—ideal for direct processing of cell-containing feedstreams.
This base is made from large-diameter (250 µm) cross-linked agarose beads functionalized with quaternary ammonium ligands (Q type). The unique bead geometry supports unhindered flow-through of cells and debris, enabling capture and clarification in a single step. ZetaCell Q operates reliably at linear velocities ≥ 2000 cm/h and system pressures up to 3 bar.
Performance Highlights
- ≥ 80% particles in the 140–360 µm range
- Linear velocity ≥ 2000 cm/h for high flow rates
- Strong anion exchanger with quaternary ammonium ligands
- Operates up to 3 bar system pressure
- pH stability: 2–14 (short term), 3–12 (long term)
- Compatible with SMART™ Chromatography for direct cell feed capture
Specifications
- Resin: Highly cross-linked beaded agarose
- Bead Diameter: Average 250 µm (≥ 80% between 140–360 µm)
- Functional Group: Quaternary ammonium (Q)
- Ionic Capacity: 0.08–0.16 mmol Cl⁻/mL resin
- Storage: +2 to +30 °C in 20% ethanol
Applications
- Strong anion exchange chromatography (AEX)
- Downstream purification of proteins and nucleic acids
- Clarified or unclarified feedstream processing
- Continuous or batch capture workflows
Why Choose ZetaCell Q in SMART™ Columns?
ZetaCell Q offers robust, high-speed anion exchange purification with strong base stability and exceptional flow dynamics. Optimized for SMART™ columns, it enables seamless process integration without clogging or pressure buildup—delivering scalable, reproducible results in bioprocessing environments.
