Description
SMART™ IEX Purification with Optimized Weak Cation Exchange Resin
ZetaCell CM Boost from emp BIOTECH is a large-bead, cross-linked agarose resin functionalized with carboxymethyl (CM) weak cation ligands. Designed specifically for SMART™ Chromatography, this resin supports direct processing of cell-containing feedstocks by enabling unobstructed flow through its open, macroporous structure.
By increasing the surface accessibility of its CM ligands, ZetaCell CM Boost delivers enhanced binding capacity without compromising flow properties. It performs reliably in continuous or batch ion exchange chromatography and is compatible with a broad pH range and common aqueous buffers.
Performance Highlights
- Ion exchange capacity: 0.08–0.22 mmol H⁺/mL
- ≥ 80% particle size in 140–360 µm range
- Linear velocity ≥ 2000 cm/h
- Operates up to 3 bar system pressure
- Broad pH stability: 2–14 (short term), 3–12 (long term)
- Hydrophilic, cross-linked agarose optimized for SMART™ formats
Specifications
- Matrix: Highly cross-linked beaded agarose
- Ligand: Carboxymethyl (CM), weak cation exchanger
- Bead Diameter: Average 250 µm
- Storage Buffer: 20% ethanol
Applications
- Weak cation exchange (CEX) chromatography
- Purification of biomolecules and protein isoforms
- Separation of acidic proteins and charge variants
- High-throughput and continuous bioprocessing
Why Choose ZetaCell CM Boost?
ZetaCell CM Boost increases productivity through enhanced ligand accessibility while maintaining all the benefits of SMART™ Chromatography—unclogged flow paths, high mechanical strength, and reliable operation with complex, unclarified feedstreams. Its design is ideal for demanding IEX separations in modern bioprocess workflows.
