Description
High-Performance SMART™ IEX with Weak Cation Exchange Resin
ZetaCell CM from emp BIOTECH is a weak cation exchange agarose resin specifically designed for SMART™ ion exchange chromatography. This cross-linked beaded agarose is surface-modified with a carboxymethyl (CM) ligand, enabling efficient separation of proteins and biomolecules under mild conditions. Its large bead size (250 µm) allows unhindered flow of cell-containing feedstocks, making it ideal for continuous bioprocessing and high-throughput chromatography.
Engineered for SMART™ columns, ZetaCell CM supports high flow rates (≥ 2000 cm/h), operates at pressures up to 3 bar, and provides stable performance across a broad pH range. Its superior physical and chemical stability ensures consistent purification, extended column lifetime, and compatibility with most aqueous buffers.
Performance Highlights
- ≥ 80% particles in the 140–360 µm range
- Linear velocity ≥ 2000 cm/h
- Operates efficiently at up to 3 bar
- Ion exchange capacity: 0.08–0.16 mmol H⁺/mL
- Broad pH stability: 2–14 (short term), 3–12 (long term)
- Hydrophilic, highly cross-linked beaded agarose
- Designed for SMART™ chromatography
Specifications
- Matrix: Cross-linked beaded agarose
- Ligand: Carboxymethyl (CM), weak cation exchanger
- Bead Diameter: Average 250 µm
- Storage Buffer: 20% ethanol
- Storage: +2 to +30 °C
Applications
- Weak cation exchange (CEX) chromatography
- Purification of biomolecules and proteins
- Separation of isoforms, charged species, or acidic proteins
- Continuous or perfusion-based bioprocessing
Why Choose ZetaCell CM in a SMART™ Column?
ZetaCell CM combines the selectivity of weak cation exchange with the clog-resistant geometry and base stability of SMART™ resin design. Ideal for mid- to large-scale purification workflows, it streamlines downstream processing with high throughput, broad buffer compatibility, and excellent performance in cell-containing feeds.
