Description
SMART™ Cation Exchange Chromatography with Enhanced Binding
ZetaCell SP Boost from emp BIOTECH is a strong cation exchange agarose resin designed for efficient protein purification in SMART™ ion exchange chromatography. Engineered with highly cross-linked 250 µm agarose beads and functionalized with sulphopropyl (SP) ligands, this resin offers superior flow performance and effective separation of positively charged biomolecules—even in unclarified feedstreams.
Thanks to increased surface-localized ligand density, ZetaCell SP Boost provides enhanced binding capacity and consistent performance across a wide pH and pressure range. It enables direct processing of complex samples in continuous or perfusion workflows, maintaining robustness and reproducibility throughout repeated cycles.
Performance Highlights
- ≥ 80% particles in the 140–360 µm range
- Linear velocity ≥ 2000 cm/h
- Operates efficiently at up to 3 bar pressure
- Ionic capacity: 0.14–0.26 mmol H⁺/mL
- Broad pH stability: 2–14 (short term), 3–12 (long term)
- Surface-modified to enable SMART™ purification of crude or cell-loaded feeds
Specifications
- Matrix: Cross-linked beaded agarose
- Ligand: Sulphopropyl (SP), strong cation exchanger
- Bead Diameter: Average 250 µm
- Storage Buffer: 20% ethanoll
Applications
- Strong cation exchange (CEX) chromatography
- Separation of proteins and biomolecules with positive net charge
- Purification from clarified and unclarified feedstocks
- Continuous downstream bioprocessing and polishing steps
Why Choose ZetaCell SP Boost?
ZetaCell SP Boost combines the reliability of strong cation exchange with the scalability and pressure resilience of SMART™ resin architecture. The large particle size ensures high flow and low backpressure, even in cell-rich samples, while enhanced ligand accessibility increases productivity in demanding purification scenarios. It is the ideal choice for robust mid- to large-scale CEX workflows where throughput and consistency matter.
