Description
High-Performance SMART™ IEX with Weak Cation Exchange Resin
ZetaCell SP from emp BIOTECH is a robust strong cation exchange (CEX) resin specifically developed for SMART™ ion exchange chromatography. Based on 250 µm cross-linked agarose beads derivatized with sulphopropyl (SP) ligands, it supports efficient binding of positively charged biomolecules under a range of process conditions. Its macro-bead format enables unhindered flow of cell-containing feedstocks—ideal for continuous bioprocessing and scalable separations.
Engineered for radial flow columns, ZetaCell SP tolerates high flow rates (≥ 2000 cm/h), operates under pressures up to 3 bar, and is chemically stable across a wide pH range. Its physical robustness and hydrophilic surface make it suitable for demanding bioprocessing applications where performance, lifetime, and flexibility are critical.
Performance Highlights
- ≥ 80% of particles in the 140–360 µm range
- Linear flow velocity ≥ 2000 cm/h
- Operates efficiently up to 3 bar system pressure
- Ion exchange capacity: 0.14–0.26 mmol H⁺/mL resin
- pH stability: 2–14 (short term), 3–12 (long term)
- Large-pore, clog-resistant agarose beads
- Compatible with SMART™ Chromatography for cell-containing feedstocks
Specifications
- Matrix: Highly cross-linked beaded agarose
- Ligand: Sulphopropyl (SP), strong cation exchanger
- Bead Diameter: Average 250 µm
- Storage Buffer: 20% ethanol
- Storage: +2 to +30 °C
Applications
- Strong cation exchange (CEX) purification
- Separation of proteins and biomolecules by charge
- Polishing of acidic biomolecules
- Continuous bioprocessing with unclarified feeds
Why Choose ZetaCell SP?
ZetaCell SP combines strong cation exchange functionality with SMART™ resin architecture, enabling high-resolution purification directly from complex or particulate-rich feedstocks. Its bead size and chemical stability support efficient, scalable workflows with high throughput and minimal clogging. Ideal for process development and large-scale manufacturing.
