Description
Salt-Tunable Protein Purification with Phenyl-Based HIC Resin
Zetarose Phenyl FF6 from emp BIOTECH is a cross-linked 6% agarose resin designed for hydrophobic interaction chromatography (HIC) of proteins and other biomolecules. Functionalized with aromatic phenyl ligands (C₆H₅), the resin enables salt-dependent interactions with hydrophobic patches on the target molecule, supporting gentle, tunable purification under native conditions.
The highly cross-linked agarose matrix with an average particle diameter of 100 µm ensures excellent pressure resistance and flow performance, while the phenyl ligand provides intermediate hydrophobicity ideal for mid-range retention. Zetarose Phenyl FF6 is suitable for scalable workflows and a wide range of downstream purification applications, including antibody polishing, virus-like particle (VLP) isolation, and enzyme separation.
Performance Highlights
- Aromatic phenyl ligand (-C₆H₅) for controlled hydrophobic interactions
- 100 µm cross-linked agarose particles for robust pressure tolerance
- Salt-dependent binding for native condition separations
- Compatible with gradient or stepwise elution
- Scalable from lab to production
- Stable in commonly used aqueous buffers
Specifications
- Matrix: 6% cross-linked beaded agarose
- Ligand: Phenyl (-C₆H₅)
- Average Bead Size: 100 µm
- Ligand Density: ~20 µmol/mL resin
- Maximum Flow Rate: >500 cm/h
Applications
- Polishing of monoclonal antibodies and proteins
- Separation of biomolecules based on surface hydrophobicity
- Intermediate or final step in purification workflows
- VLP and enzyme purification under mild, non-denaturing conditions
Why Choose Zetarose Phenyl FF6?
Zetarose Phenyl FF6 offers robust, reproducible performance in HIC workflows that require reliable retention and gentle elution. Its intermediate hydrophobicity, excellent flow properties, and scalable design make it an excellent choice for bioprocess engineers and scientists seeking efficient separation without compromising biomolecular structure or activity.
