Description
Flexible HIC Resin for Protein Separation in High-Salt Conditions
Zetarose Butyl FF6 from emp BIOTECH is a hydrophobic interaction chromatography (HIC) resin designed for efficient purification of proteins and biomolecules based on hydrophobicity. This 6% cross-linked agarose resin is derivatized with a short-chain aliphatic butyl ligand (C₄H₉), providing moderate hydrophobic interaction ideal for gradient elution workflows.
The 100 µm beaded agarose structure offers excellent pressure resistance and flow performance, enabling high-throughput purification without compromising resolution. Zetarose Butyl FF6 is particularly suitable for capturing proteins under high-salt conditions and eluting under decreasing salt gradients, offering reproducible performance for a variety of downstream processing steps.
Performance Highlights
- 6% cross-linked agarose for improved rigidity and pressure resistance
- Ligand: butyl (C₄H₉) for moderate hydrophobic interaction
- Average bead diameter: 100 µm (45–160 µm range)
- Ligand density: 40 µmol/mL resin
- Maximum flow rate: > 500 cm/h (32 mm ID column)
- Autoclavable at 121 °C for 20 minutes
Specifications
- Matrix: Highly cross-linked agarose
- Functional Group: Aliphatic butyl
- Bead Diameter: 100 µm average
- pH Stability: Compatible with standard aqueous buffers
- Storage: +2 to +30 °C in 20% ethanol
- Format: Bulk resin; ZetaSep FPLC columns available
Applications
- Protein purification based on hydrophobicity
- Capture and intermediate steps in HIC workflows
- Sample fractionation under controlled salt gradient
- Purification of proteins with moderate hydrophobicity
Why Choose Zetarose Butyl FF6?
Zetarose Butyl FF6 offers a balanced HIC solution for reliable protein purification with strong mechanical stability and scalable process compatibility—ideal for researchers optimizing hydrophobic interaction strategies in mid- to large-scale purification workflows.
