Description
Hydrophobic Protein Purification with Small-Sized Phenyl Agarose Resin
Zetarose Phenyl HC from emp BIOTECH is a small-sized, 6% cross-linked beaded agarose resin engineered for hydrophobic interaction chromatography (HIC) under conditions where high resolution and strong hydrophobic selectivity are essential. Immobilized with aromatic phenyl ligands, this resin enables efficient separation of proteins and biomolecules through controlled hydrophobic interactions modulated by salt concentration.
With an average bead diameter of 30 µm and high ligand density (40 µmol/mL), Zetarose Phenyl HC delivers reliable performance at lower flow rates—ideal for polishing steps, protein isoform resolution, or process optimization in research and production workflows.
Performance Highlights
- 30 µm highly cross-linked agarose beads for high-resolution separations
- 40 µmol phenyl ligand per mL resin
- Robust flow rate capacity: >100 cm/h (32 mm column ID)
- Salt-dependent protein retention for tunable selectivity
- Stable under aqueous buffer conditions
- Reproducible results with native or partially denatured proteins
Specifications
- Matrix: 6% cross-linked beaded agarose
- Functional Group: Aromatic phenyl (C₆H₅)
- Bead Diameter: 30 µm
- Ligand Density: ~40 µmol/mL resin
- pH Stability: 3–12
- Max Flow Rate: >100 cm/h (tested in 32 mm column ID)
Applications
- Hydrophobic interaction chromatography (HIC)
- Protein polishing and isoform separation
- Intermediate purification in bioprocess workflows
- Optimization of salt-gradient based separations
Why Choose Zetarose Phenyl HC?
Zetarose Phenyl HC offers a balance of fine particle size and high ligand density, making it an ideal choice when separation resolution is more critical than throughput. Its 30 µm beads enhance surface area and resolution, supporting fine-tuned hydrophobic separations for protein purification and formulation development.
