Description
Selective Purification of His-Tagged Proteins with IMAC Resin
Zetarose Ni-NTA FF6 from emp BIOTECH is a 6 % cross-linked beaded agarose resin functionalized with nickel-charged nitrilotriacetic acid (Ni-NTA) for efficient immobilized metal affinity chromatography (IMAC). Specifically designed for purification of polyhistidine (His)-tagged proteins, this resin supports high binding capacity, low non-specific binding, and flexible elution options under both native and denaturing conditions.
The resin’s agarose matrix offers excellent physical stability and flow performance at up to 3 bar, with consistent results in manual and automated workflows. Zetarose Ni-NTA FF6 is ideal for reproducible affinity capture of His-tagged proteins from clarified lysates or fermentation broths.
Performance Highlights
- ≥ 40 mg His-tagged protein/mL resin (SBC)
- ≥ 20 µmol Ni²⁺/mL resin
- Compatible with imidazole, histidine, or low-pH elution
- Stable at ≥ 500 cm/h flow rates
- Operates at up to 3 bar system pressure
- For native or denaturing purification protocols
Specifications
- Matrix: 6 % cross-linked agarose
- Functional Group: Ni²⁺-NTA (nitrilotriacetic acid)
- Average Bead Size: 100 µm
- Storage Buffer: 20 % ethanol
Applications
- Affinity purification of polyhistidine (6xHis)-tagged recombinant proteins
- Native and denaturing IMAC workflows
- Batch and column chromatography formats
Why Choose Zetarose Butyl FF6?
Zetarose Ni-NTA FF6 delivers high binding capacity and robust performance for His-tagged protein purification. With excellent selectivity and reproducibility, it ensures effective capture and elution with minimal optimization—ideal for scalable, high-yield IMAC processes.
