Description
Affinity Chromatography Resin for Versatile IMAC with Metal-Free NTA
Zetarose NTA FF6 from emp BIOTECH is a 6 % cross-linked beaded agarose resin derivatized with metal-free nitrilotriacetic acid (NTA), enabling flexible immobilized metal affinity chromatography (IMAC) for the purification of His-tagged proteins. This uncharged version allows users to pre-load the resin with their metal ion of choice (Ni²⁺, Co²⁺, Cu²⁺, or Zn²⁺), tailoring affinity and specificity to the needs of each application.
The 100 µm beads provide an optimal balance of flow performance and surface area, while the stable resin matrix supports native or denaturing conditions. Zetarose NTA FF6 is ideal for research, process development, or customized screening workflows involving recombinant proteins with polyhistidine (6xHis) tags.
Performance Highlights
- Flexible metal-ion charging for custom selectivity
- Efficient purification of 6xHis-tagged proteins
- Compatible with Ni²⁺, Co²⁺, Cu²⁺, and Zn²⁺
- Suitable for use under native or denaturing conditions
- Operates efficiently at ≥ 500 cm/h and ≤ 3 bar
- Stable cross-linked 100 µm agarose beads
Specifications
- Matrix: Cross-linked 6 % beaded agarose
- Ligand: Nitrilotriacetic acid (NTA), metal-free
- Bead Diameter: Average 100 µm
- pH Stability: 3–13 (dependent on metal ion and elution conditions)
Applications
- Purification of polyhistidine-tagged recombinant proteins
- Metal-ion selectivity studies and optimization
- IMAC under native or denaturing conditions
- Research and development of customized protein workflows
Why Choose Zetarose Phenyl FF6?
Zetarose NTA FF6 offers maximum flexibility for affinity purification of His-tagged proteins by allowing on-demand metal ion loading. Its robust, high-performance matrix ensures reproducibility and reliability in both small- and mid-scale workflows, making it a smart choice for labs seeking versatile and customizable IMAC resin solutions.
