Description
Efficient Antibody Purification with NaOH-Stable Affinity Resin
Zetarose Protein A Elevate from emp BIOTECH is a cross-linked agarose affinity resin designed for efficient purification of immunoglobulins via selective binding to a recombinant Protein A ligand. Engineered for base stability and compatibility with standard purification protocols, this resin enables fast and reliable antibody capture in gravity or pressure-driven workflows.
The resin is based on highly cross-linked 100 µm agarose beads with ≥ 80% of particles in the 45–160 µm range. Its NaOH-tolerant ligand supports repeated cleaning-in-place (CIP) cycles and stable performance in a broad pH window. Zetarose Protein A Elevate is ideal for preparative-scale IgG purification workflows requiring strong performance, reproducibility, and long resin lifetime.
Performance Highlights
- ≥ 30 mg/mL binding capacity (human IgG)
- ≥ 80% of particles in the 45–160 µm range
- Linear velocity ≥ 300 cm/h
- Stable in 1 M NaOH for up to 15 minutes
- Operates efficiently at up to 3 bar pressure
- Recombinant Protein A ligand, NaOH-tolerant
Specifications
- Matrix: Highly cross-linked beaded agarose
- Ligand: Recombinant NaOH-tolerant Protein A
- Bead Diameter: 100 µm average (≥ 80% within 45–160 µm)
- pH Stability: 2–14 (short term), 3–9 (long term)
- Storage Buffer: 20% ethanol
Applications
- Immunoglobulin G (IgG) and antibody purification
- Alkaline-tolerant affinity chromatography
- Capture from clarified lysates or unclarified feeds
- Reusable purification workflows with CIP
Why Choose Zetarose Protein A Elevate?
Zetarose Protein A Elevate provides a durable, high-yield solution for IgG capture in biomanufacturing and research workflows. Its base-stable ligand, robust particle design, and wide pH tolerance make it ideal for repeated use in stringent cleaning conditions—offering performance and reliability batch after batch.
