Description
Robust Strong Cation Exchange for High-Yield Protein Purification
Zetarose SP FF6 from emp BIOTECH is a 6% cross-linked beaded agarose resin functionalized with sulphopropyl (SP) ligands for strong cation exchange chromatography (CEX). It delivers reliable, high-capacity separation of proteins and biomolecules, even under demanding downstream purification workflows.
The solid phase is based on Zetarose FlashFlow agarose, engineered for optimal flow properties, mechanical stability, and broad chemical resistance. With particle sizes ranging from 45–160 µm and ≥ 80% in range, Zetarose SP FF6 is suitable for column packing in lab- to pilot-scale purification of positively charged proteins and enzymes.
Performance Highlights
- Strong cation exchange capacity: 0.14–0.26 mmol H⁺/mL
- Particle range: ≥ 80% between 45–160 µm
- Linear velocity: ≥ 500 cm/h
- Pressure resistance: up to 3 bar
- Broad pH stability: 2–14 (short term), 4–12 (long term)
- Chemically stable in commonly used aqueous buffers
Specifications
- Matrix: 6% highly cross-linked beaded agarose
- Ligand: Sulphopropyl (SP)
- Average Bead Diameter: 100 µm
- Storage Buffer: 0.2 M sodium acetate in 20% ethanol
Applications
- Ion exchange chromatography (CEX)
- Protein and enzyme purification
- Capture and intermediate purification steps
- Processing of biomolecules under varying pH conditions
Why Choose Zetarose SP FF6?
Zetarose SP FF6 combines strong cation exchange functionality with robust physical and chemical performance. Its uniform bead size and high binding capacity enable reproducible purification across various applications—making it an ideal solution for process development and scalable purification of positively charged proteins.
