Description
Weak Cation Exchange Chromatography with 6% Cross-Linked Agarose Resin
Zetarose CM FF6 from emp BIOTECH is a 6% cross-linked beaded agarose resin functionalized with a carboxymethyl (CM) ligand for weak cation exchange chromatography. Designed for high-resolution protein purification, this resin combines reliable binding capacity with chemical and physical robustness in a 100 µm average bead format.
Its optimized particle distribution (≥ 80% between 45–160 µm) allows stable performance in scalable workflows, from lab-scale separations to industrial processes. Zetarose CM FF6 operates at linear flow velocities ≥ 500 cm/h and tolerates pressures up to 3 bar, while maintaining integrity across a wide pH range (2–14 short-term, 4–12 long-term). Ideal for mild purification of biomolecules sensitive to pH or harsh elution conditions.
Performance Highlights
- Ion exchange capacity: 0.08–0.16 mmol H⁺/mL
- ≥ 80% of particles within 45–160 µm
- Linear velocity ≥ 500 cm/h
- Stable up to 3 bar operating pressure
- Broad pH stability (2–14 short term, 4–12 long term)
- Compatible with aqueous buffers; avoid oxidizers and cationic detergents
Specifications
- Matrix: Zetarose FlashFlow 6% cross-linked agarose
- Ligand: Carboxymethyl (CM), weak cation exchanger
- Average Bead Diameter: 100 µm
- Storage Buffer: 20% ethanol
Applications
- Weak cation exchange (CEX) chromatography
- Purification of acidic proteins and charged biomolecules
- Intermediate and polishing steps in downstream processin
Why Choose Zetarose CM FF6?
Zetarose CM FF6 delivers reproducible separations and gentle handling of proteins in weak cation exchange workflows. Its bead structure ensures low backpressure and high flow capacity, making it ideal for purification strategies requiring selectivity, scalability, and pH flexibility.
