Description
Efficient Strong Anion Exchange Chromatography for Protein Purification
Zetarose Q FF6 from emp BIOTECH is a cross-linked 6% beaded agarose resin functionalized with quaternary ammonium (Q) groups for strong anion exchange (AEX) chromatography. Designed to purify negatively charged biomolecules such as nucleic acids and acidic proteins, Zetarose Q FF6 offers reliable performance in both research and manufacturing workflows.
With an average bead diameter of 100 µm and ≥80% of particles in the 45–160 µm range, this resin delivers fast flow, low backpressure, and excellent resolution in gravity, low-pressure, or automated chromatography setups. Zetarose Q FF6 is stable over a broad pH range and operates at linear velocities ≥500 cm/h and up to 3 bar system pressure.
Performance Highlights
- Strong anion exchange (Q) ligand for broad AEX applications
- ≥80% particles in the 45–160 µm range
- Linear velocity ≥500 cm/h
- Stable up to 3 bar operating pressure
- Broad pH stability: 2–14 (short term), 3–12 (long term)
- High chemical and mechanical stability in 6% cross-linked agarose
Specifications
- Matrix: 6% cross-linked beaded agarose (Zetarose FlashFlow)
- Ligand: Quaternary ammonium (Q), strong anion exchanger
- Bead Size: Average 100 µm
- Ion Exchange Capacity: 0.08–0.16 mmol Cl⁻/mL resin
- Storage Buffer: 20% ethanol
Applications
- Anion exchange chromatography (AEX)
- Purification of nucleic acids, acidic proteins, and other negatively charged biomolecules
- Process development and manufacturing of biologics
- High-throughput and continuous downstream processing
Why Choose Zetarose Q FF6?
Zetarose Q FF6 combines high-flow performance with robust binding capacity in a stable, cross-linked agarose matrix. Its strong anion exchange chemistry and consistent particle distribution ensure high reproducibility, fast run times, and scalability from process development to manufacturing. Ideal for applications requiring reliable separation of negatively charged biomolecules under stringent or mild conditions.
