Description
Versatile Dual-Mode Gel Filtration Resin for Organic and Aqueous Systems
Zetadex-20 LH from emp BIOTECH is a hydroxypropylated gel filtration matrix combining lipophilic and hydrophilic properties for flexible use in both polar organic solvents and aqueous solvent mixtures. This dry beaded composite, based on Zetadex-25 Fine, is specifically designed for desalting and size exclusion chromatography in demanding purification environments.
The resin enables efficient separation of biomolecules by size, with a molecular weight cut-off (MWCO) around 4–5 kDa—dependent on the solvent system used. Whether you’re purifying proteins or nucleic acids, Zetadex-20 LH provides a robust and chemically stable matrix, well-suited for process-scale purification applications where standard aqueous gels may fail.
Performance Highlights
- Dual-mode compatibility: Effective in polar organic solvents and aqueous mixtures
- Size exclusion range: ~4–5 kDa (solvent-dependent)
- Dry bead diameter: 20–80 µm (>80% in range)
- Water regain: 1.90–2.10 mL/g
- Swelling capacity:
- Water: 4.0–4.2 mL/g
- Chloroform: 3.6–4.1 mL/g
- Ethanol (96%): 3.6–3.9 mL/g
- Acetone: 2.2–2.4 mL/g
Specifications
- Matrix: Hydroxypropylated cross-linked spherical dextran composite
- Bead size: 20–80 µm (dry)
- Size exclusion limit: ~4–5 kDa (solvent-dependent)
- Chemical stability: Stable in most aqueous and organic eluents
- pH range: 2–13
- Autoclavable: 121 °C, pH 7 for 30 min
- Appearance: White dry solid
Applications
- Desalting and buffer exchange in non-aqueous or hybrid solvent systems
- Size-exclusion chromatography of proteins and nucleic acids
- Ideal for purification workflows where traditional gels are incompatible
Why Choose Zetadex-20 LH?
Zetadex-20 LH offers unmatched flexibility for researchers and process engineers working in mixed solvent systems. Its dual-mode compatibility, consistent bead sizing, and high-performance exclusion characteristics make it a go-to solution for challenging desalting and separation tasks in both research and manufacturing environments.
