Description
Pre-Hydrated Size-Exclusion Resin for Efficient Desalting and Buffer Exchange
Zetadex-25 Fine, Hydrated in Deionized Water / 0.15% ProClin is a ready-to-use size-exclusion chromatography (SEC) resin developed by emp BIOTECH for rapid and efficient desalting, buffer exchange, and small molecule removal. This hydrated gel filtration matrix features fine, cross-linked dextran beads with a dry particle size range of 20–80 µm, optimized for reliable performance in purification processes of proteins and nucleic acids.
The resin is pre-hydrated in deionized water and stabilized with 0.15% ProClin, ensuring convenience and consistency in daily lab workflows. Like all Zetadex-25 products, this formulation offers high resolution, low nonspecific adsorption, and robust chemical stability.
Performance Highlights
- Pre-hydrated and ready to use
- Bead diameter (dry): 20–80 µm
- Molecular weight cut-off (MWCO): ~5 kDa for proteins, ~10 base pairs for nucleic acids
- Buffer compatibility: Compatible with a wide range of aqueous buffers
Specifications
- Resin Type: Zetadex-25 Fine
- Bead Diameter (dry): 20–80 µm
- Hydration Medium: Deionized water with 0.15% ProClin
- Size Exclusion Cut-Off: ~5 kDa for proteins, ~10 bp for nucleic acids
- Fractionation Range: 1–5 kDa (globular proteins)
- Water Regain: 2.15–2.25 mL/g
- Swelling Factor: 4–6 mL/g
Applications
- Desalting of proteins, nucleic acids, and nanoparticles
- Rapid buffer exchange
- Removal of dyes, salts, and other small contaminants
Why Choose Zetadex-25 Fine (Hydrated in Water / ProClin)?
This hydrated formulation combines the performance of Zetadex-25 Fine with the convenience of immediate usability. With a sharp size exclusion threshold and compatibility with a broad range of biomolecules, it ensures reproducible results for demanding desalting and buffer exchange workflows—without the need for pre-swelling or buffer equilibration.
