Description
Efficient Ready-to-Use Desalting with Pre-Hydrated Gel Filtration Resin
Zetadex-50 Medium, Hydrated in Deionized Water / 0.15% ProClin (Product No. TM-0129) is a pre-hydrated gel filtration matrix designed for rapid purification, desalting, and buffer exchange of proteins and nucleic acids. Made from cross-linked dextran, this medium-grade size-exclusion resin is ideal for gravity-flow applications in process purification workflows.
With a dry bead size of 50–150 µm and a well-defined molecular weight cut-off, it efficiently separates biomolecules based on size—retaining proteins and nucleic acids while removing salts, dyes, and other low-molecular-weight contaminants. This ready-to-use formulation in deionized water and 0.15% ProClin offers added convenience and consistency for routine processing.
Performance Highlights
- Pre-hydrated resin in deionized water / 0.15% ProClin
- Ready-to-use for immediate application
- MWCO (Size exclusion limit): ~25 kDa for proteins, ~20 base pairs for nucleic acids
- High resolution and chemical stability
- Minimal buffer and pH sensitivity
- Optimized for gravity flow
Specifications
- Bead Diameter (dry): 50–150 µm
- Resin Composition: Cross-linked dextran composite
- Water Regain: 4.80–5.20 mL/g
- Swelling Capacity: 9–11 mL/g
- Molecular Weight Cut-Off: ~25 kDa for proteins / ~20 bp for nucleic acids
- Fractionation Range: 1–30 kDa (globular proteins)
- Storage Buffer: Deionized water with 0.15% ProClin
Applications
- Desalting of proteins and nucleic acids
- Buffer exchange prior to ion exchange, HIC, or affinity chromatography
- Removal of low-molecular-weight impurities (e.g., salts, dyes)
- Sample cleanup before downstream analysis (e.g., electrophoresis, MS)
- Gravity flow, batch, or spin column workflows
Why Choose Zetadex-50 Medium (Hydrated)?
Zetadex-50 Medium offers a perfect balance between flow properties and resolution. The hydrated formulation in deionized water with ProClin provides excellent convenience, while the well-controlled bead size ensures reproducibility. Its strong performance in desalting and buffer exchange makes it an essential tool for downstream biomolecule processing.
