Description
High-Resolution Size Exclusion for Small-Scale Protein & Nucleic Acid Purification
Zetadex-50 Superfine, Hydrated in Deionized Water / 0.15% ProClin is a ready-to-use gel filtration matrix developed by emp BIOTECH for rapid and efficient desalting, buffer exchange, and small molecule removal. Based on a 20–50 µm beaded composite of ultrapure cross-linked dextran, this pre-hydrated resin offers excellent resolution and minimal sample dilution in small-volume workflows.
Optimized for high-resolution size exclusion chromatography, Zetadex-50 Superfine separates molecules based on size, allowing larger proteins and nucleic acids to elute first while retaining salts, dyes, and other low molecular weight contaminants within the gel. Its pre-hydration in deionized water with 0.15% ProClin ensures immediate use in gravity, spin, or low-pressure column formats.
Performance Highlights
- Pre-hydrated and ready to use: suspended in deionized water with 0.15% ProClin
- Bead diameter: 20–50 µm (dry)
- Size exclusion cut-off:
- ~25 kDa for proteins
- ~20 base pairs for nucleic acids
- Swelling ratio: 4–6 mL/g
- Water regain: 4.8–5.2 mL/g
- Stable in commonly used aqueous buffers
- Compatible with centrifugation, peristaltic pump, or automated systems
Specifications
- Matrix: Cross-linked dextran composite
- Bead size (dry): 20–50 µm (>80%)
- Storage solution: Deionized water + 0.15% ProClin
- Molecular Weight Cut-Off (MWCO): ~25 kDa (proteins), ~20 bp (nucleic acids)
- Recommended application: High-resolution desalting and buffer exchange
Applications
- Desalting of protein and nucleic acid solutions
- Rapid removal of small molecules (e.g., salts, dyes, biotin)
- Buffer exchange into aqueous systems
Why Choose Zetadex-50 Superfine, Hydrated?
Zetadex-50 Superfine delivers exceptional resolution for size exclusion chromatography, thanks to its narrow particle size range and cross-linked dextran-based composite matrix. Pre-hydrated in deionized water with 0.15% ProClin, the resin eliminates preparation steps and enables immediate use in desalting, buffer exchange, or small-molecule removal workflows. Its consistent performance across formats—from spin columns to automated systems—makes it a dependable solution for high-purity biomolecule processing.
