Description
Ready-to-Use Size-Exclusion Gel for Efficient Biomolecule Purification
Zetadex-50 Superfine, Hydrated in PBS / 0.02% NaN₃ (TM-0121) is a pre-hydrated gel filtration matrix developed by emp BIOTECH. It consists of highly purified, cross-linked dextran beads optimized for rapid purification, desalting, and buffer exchange of proteins and nucleic acids. Pre-suspended in PBS and autoclaved for sterility, this matrix is ready to use in gravity flow or chromatography workflows without the need for further preparation.
This superfine grade delivers excellent resolution and separation performance with minimal sample dilution. It is ideal for purifying proteins ≥ 25 kDa and nucleic acids ≥ 20 bp.
Performance Highlights
- Pre-hydrated in PBS (pH 7.4, 0.02% NaN₃) and autoclaved for immediate use
- Size exclusion cut-off: ~25 kDa for proteins, ~20 bp for nucleic acids
- Bead size (dry): 20–50 µm (wet: 40–100 µm)
- Fractionation range: 1,500–30,000 Da (globular proteins)
- Water regain: 4.80–5.20 mL/g
- Swelling capacity: 9–11 mL/g
- Autoclavable: 121 °C, pH 7 for 30 minutes
- pH stability: 2–10
- Chemically stable in common buffers, NaOH, HCl, urea, guanidine, SDS
Specifications
- Matrix: Cross-linked spherical dextran composite
- Dry Bead Size: 20–50 µm
- Wet Bead Size: 40–100 µm
- Fractionation Range: 1.5–30 kDa (proteins), 0.5–10 kDa (dextrans)
- Preservative: None (autoclaved)
- Recommended Flow Rate: As high as possible without deforming beads
- Maximum Pressure: Up to 3 bar
Applications
- Desalting of protein and DNA/RNA solutions
- Buffer exchange prior to downstream analysis
- Rapid separation of high- and low-molecular-weight species
- Preparation of pure biomolecule fractions for analytical or preparative use
Why Choose Zetadex-50 Superfine, Hydrated in PBS?
Zetadex-50 Superfine, hydrated in PBS, provides immediate usability, exceptional separation precision, and robust buffer compatibility. Its ready-to-use format saves preparation time and ensures reproducibility in demanding workflows, especially when fast and reliable desalting or buffer exchange is essential.
