Description
Reliable Desalting with Pre-Hydrated Size Exclusion Resin for Routine Purification
Zetadex-25 Medium, Hydrated in Deionized Water / 0.15% ProClin™ from emp BIOTECH is a ready-to-use size exclusion chromatography (SEC) matrix designed for efficient desalting and buffer exchange of proteins and nucleic acids. Based on cross-linked dextran, the resin offers excellent chemical stability, narrow particle size distribution, and consistent resolution. The hydrated format saves preparation time and simplifies integration into purification workflows for bioprocessing, research, and diagnostic applications.
Molecules are separated according to size, with larger biomolecules eluting earlier than smaller ones. This allows for the rapid removal of salts, dyes, and other small molecules without significant dilution of the target protein or nucleic acid.
Performance Highlights
- Pre-hydrated for immediate use in desalting workflows
- Bead size: 50–150 µm (dry)
- Size exclusion limit: ~5 kDa for proteins, ~10 bp for nucleic acids
- Fractionation range for proteins: 1–5 kDa
- Chemically stable and reusable in standard buffer systems
- Compatible with gravity-flow columns or low-pressure systems
Specifications
- Matrix: Composite cross-linked dextran
- Bead Diameter: 50–150 µm (dry)
- Water Regain: 2.15–2.25 mL/g
- Swelling: 4–6 mL/g
- Hydration Buffer: Deionized water with 0.15% ProClin™
- Format: Bulk powder or pre-packed columns
Applications
- Desalting and buffer exchange of proteins and nucleic acids
- Removal of small molecules (<5 kDa) prior to downstream processing
- Sample cleanup for electrophoresis, chromatography, or labeling steps
Why Choose Zetadex-25 Medium, Hydrated in Water / 0.15% ProClin™?
Zetadex-25 Medium combines speed, ease of use, and reproducibility in desalting applications. Its hydrated, ready-to-use format reduces setup time while delivering precise separation performance. Whether for routine lab-scale purification or diagnostic workflows, this resin offers robust and consistent results with minimal effort.
