Description
High-Resolution Gel Filtration for Rapid Desalting in PBS
Zetadex-50 Fine, Hydrated in PBS / 0.02% NaN₃ is a ready-to-use gel filtration resin optimized for high-resolution desalting and buffer exchange of proteins and nucleic acids. Developed by emp BIOTECH, the resin features a pre-hydrated beaded composite matrix of cross-linked dextran suspended in phosphate-buffered saline with 0.02% sodium azide. Its fine particle size (20–80 µm) ensures excellent resolution while enabling convenient processing of samples via gravity flow, centrifugation, or liquid chromatography systems.
This variant is ideal for laboratories requiring rapid, high-throughput purification of biomolecules without the need for rehydration or equilibration, and for workflows where compatibility with PBS is critical.
Performance Highlights
- Pre-hydrated in PBS with 0.02% NaN₃: Immediate use without rehydration
- Bead size: 20–80 µm (dry)
- MWCO (Size exclusion limit): ~25 kDa for proteins and ~20 base pairs for nucleic acids
- Fractionation range: 1–30 kDa for globular proteins
- Chemical stability: Compatible with standard biological buffers
- Swelling: 9–11 mL/g
- Water regain: 4.8–5.2 mL/g
Specifications
- Matrix: Cross-linked dextran (Zetadex-50 Fine)
- Bead diameter (dry): 20–80 µm
- Hydration medium: Phosphate buffered saline (PBS) pH 7.4, with 0.02% NaN₃
- Ready-to-use: Yes, no rehydration needed
- Autoclavable: 121 °C at pH 7 for 30 minutes
- pH range stability: Broad compatibility with most purification conditions
Applications
- Desalting of protein and nucleic acid solutions
- Buffer exchange into PBS
- Rapid removal of salts, dyes, small molecule impurities
- Sample preparation for downstream processing or labeling
- Suitable for use with gravity flow, syringes, peristaltic pumps, and automated LC systems
Why Choose Zetadex-50 Fine in PBS?
Zetadex-50 Fine (hydrated in PBS) offers the convenience of a pre-equilibrated resin combined with the trusted performance of emp BIOTECH’s size-exclusion technology. The fine particle size ensures excellent resolution, and its compatibility with a broad range of biomolecules makes it ideal for labs requiring reproducibility, speed, and precision in purification workflows.
