Description
Pre-Hydrated Gel Filtration for Ready-to-Use Desalting and Buffer Exchange
Zetadex-25 Medium in PBS / 0.02% NaN₃ (TM-0107) is a pre-hydrated size-exclusion gel filtration matrix developed by emp BIOTECH for rapid purification of proteins and nucleic acids. The resin is based on cross-linked dextran beads, precision-sized and suspended in phosphate-buffered saline for immediate use—no swelling or equilibration required.
This medium-grade gel resin offers high selectivity and resolution with minimal non-specific binding. It is ideal for gravity-flow desalting and buffer exchange, especially for larger biomolecules such as antibodies, enzymes, and nucleic acids.
Performance Highlights
- Ready-to-use format: Pre-hydrated in PBS with 0.02% sodium azide for convenience
- High resolution: Uniform bead size for sharp separation profiles
- Fast and efficient: Designed for rapid removal of small molecules and buffer exchange
- Excellent compatibility: Stable in a wide range of buffer systems and pH values
- Low dilution: Purified biomolecules retain concentration
Specifications
- Bead structure: Cross-linked spherical dextran composite
- Dry bead diameter: 50–150 µm (>80%)
- Wet bead diameter: 85–260 µm
- Water regain: 2.15–2.25 mL/g
- Swelling factor: 4–6 mL/g
- Size exclusion cut-off: ~5 kDa (proteins), ~10 base pairs (nucleic acids)
- pH stability: 2.0–13.0
- Chemical compatibility: Stable in NaOH, HCl, acetic acid, urea, guanidine HCl, SDS, ethanol, propanol, acetonitrile
- Autoclavable: Yes, at 121 °C, pH 7 for 30 min
- Appearance: White solid suspension
- Storage buffer: PBS (pH 7.4) with 0.02% NaN₃
Applications
- Desalting of protein and nucleic acid samples
- Rapid buffer exchange for sample preparation
- Removal of low molecular weight impurities prior to downstream applications
Why Choose Zetadex-25 Medium, Hydrated in PBS?
Zetadex-25 Medium Hydrated in PBS delivers convenience and performance in a ready-to-use format, eliminating the need for pre-swelling and equilibration. Its optimized particle size ensures consistent flow and high-resolution separation, making it ideal for lab-scale desalting and buffer exchange workflows. With robust chemical stability and broad buffer compatibility, this pre-hydrated resin simplifies your purification process without compromising on purity or yield.
