Description
High-Resolution Gel Filtration for Protein and Nucleic Acid Purification
Zetadex-50 Fine from emp BIOTECH is a dry powder, size-exclusion chromatography resin designed for high-resolution purification of proteins and nucleic acids. Composed of a cross-linked dextran composite, the resin features a dry bead diameter of 20–80 µm and delivers excellent separation based on molecular size.
The resin provides superior selectivity and resolution while maintaining chemical and pH stability across a broad range of conditions. Its narrow particle size distribution allows efficient desalting and buffer exchange without significant sample dilution.
Performance Highlights
- Bead diameter (dry): 20–80 µm
- Bead diameter (wet): 40–160 µm
- Water regain: 4.8–5.2 mL/g
- Swelling: 9–11 mL/g
- Maximum pressure: 3 bar
- pH stability: 2.0–10.0
- Chemical compatibility: Stable in 0.2 M NaOH, 0.2 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1% SDS, 24% ethanol, 30% propanol, 30% acetonitrile
- Autoclavable: 121 °C at pH 7 for 30 minutes
Specifications
- Size Exclusion Cut-off (MWCO): ~25 kDa (proteins), ~20 bp (nucleic acids)
- Size Exclusion Range: 3–50 kDa (globular proteins)
- Water regain: 4.8–5.2 mL/g
- Swelling capacity: 9–11 mL/g
- Maximum operating pressure: 3 bar
- pH stability range: 2.0–10.0
- Autoclavability: 121 °C, pH 7, for 30 minutes
- Appearance: Pale yellow to white, dry powder
Applications
- Desalting of protein and nucleic acid samples
- Buffer exchange in bioprocess workflows
- Size-based separation in downstream purification
- High-resolution processing for sensitive biomolecules
Why Choose Zetadex-50 Fine?
Zetadex-50 Fine combines the precision of narrow bead size with excellent resolution and broad chemical compatibility. Its ability to maintain performance under harsh conditions makes it an optimal choice for high-resolution desalting and buffer exchange steps in biomanufacturing and research workflows.
