Description
Precision Gel Filtration with High-Resolution Dextran Composite
Zetadex-50 Superfine, developed by emp BIOTECH, is a high-resolution gel filtration matrix composed of cross-linked spherical dextran beads. With a narrow dry bead size distribution of 20–50 µm, this dry powder matrix is optimized for precise size-exclusion chromatography of proteins and nucleic acids in process-scale applications.
The resin provides excellent resolution, chemical robustness, and minimal buffer or pH impact on separation quality. Designed for professional manufacturing and laboratory use, Zetadex-50 Superfine is ideal for purifying biomolecules ranging from peptides to larger proteins and nucleic acid fragments. Its performance characteristics make it suitable for desalting, buffer exchange, and gentle biomolecule separation in aqueous systems.
Performance Highlights
- Size-exclusion cut-off: ~25 kDa for proteins, ~20 bp for nucleic acids
- Dry bead size: 20–50 µm (≥ 80% in range)
- Wet bead size: 40–100 µm
- Stable in 0.2 M NaOH, HCl, acetic acid, urea, guanidine HCl, SDS, ethanol, and organic solvents
- pH stability range: 2.0–10.0
- Autoclavable at 121 °C, pH 7 for 30 minutes
- Excellent resolution with minimal dilution of purified biomolecules
- Compatible with pressures up to 3 bar
Specifications
- Matrix: Cross-linked spherical dextran composite
- Bead Diameter (dry): 20–50 µm
- Bead Diameter (wet): 40–100 µm
- Water Regain: 4.80–5.20 mL/g
- Swelling Volume: 9–11 mL/g
- Appearance: White solid
- Operating Pressure Model: Ko = 13.5 (Darcy’s Law)
- CAS Number: 9048-71-9
Applications
- Desalting and buffer exchange
- Size-based purification of proteins and nucleic acids
- High-resolution polishing steps in downstream processing
Why Choose Zetadex-50 Superfine?
Zetadex-50 Superfine offers exceptional resolution and reliability for demanding gel filtration tasks. Its precisely defined bead structure and chemical resilience ensure reproducibility and robust performance in both research and industrial environments. Ideal for applications requiring gentle separation without sacrificing purity or yield.
