Description
High-Resolution Gel Filtration for Sensitive Biomolecule Separation
Zetadex-25 Superfine from emp BIOTECH is a dry, cross-linked dextran-based gel filtration matrix optimized for high-resolution separation of proteins and nucleic acids by size exclusion. With a bead diameter of 20–50 µm (dry), this superfine resin enables precise purification, minimizing buffer and pH interference while ensuring strong selectivity and excellent chemical resistance.
Designed for use in process chromatography and analytical desalting workflows, Zetadex-25 Superfine supports reproducible performance even under demanding conditions. Its bead uniformity and stability make it ideal for separating small to medium biomolecules without significant dilution, making it a preferred choice for sensitive downstream purification.
Performance Highlights
- Size exclusion cut-off: ~5 kDa for proteins, ~10 bp for nucleic acids
- Narrow bead size range: 20–50 µm (dry), 35–90 µm (hydrated)
- High resolution and excellent selectivity
- Stable in pH 2–13 and in solvents such as 0.2 M NaOH, 6 M guanidine HCl, 30% acetonitrile
- Autoclavable at 121 °C (pH 7, 30 min)
- Compatible with up to 3 bar operating pressure
- Easy to hydrate, pack, and use across formats
Specifications
- Matrix: Cross-linked spherical dextran composite
- Bead Diameter (dry): 20–50 µm
- Bead Diameter (wet): 35–90 µm
- Water Regain: 2.15–2.25 mL/g
- Swelling Capacity: 4–6 mL/g
- Appearance: White powder
- Chemical Compatibility: 0.2 M NaOH, 0.2 M HCl, 8 M urea, 24% EtOH, and more
- pH Stability: 2.0–13.0
- Autoclavable: Yes, at 121 °C for 30 min
Applications
- Desalting of sensitive proteins and nucleic acids
- Size-exclusion chromatography in process development
- Buffer exchange for biomolecules in small-scale workflows
- High-resolution purification of analytes with minimal sample dilution
Why Choose Zetadex-25 Superfine?
Zetadex-25 Superfine offers exceptional resolution and chemical stability for sensitive size-based separations. Its superfine, cross-linked dextran beads provide consistent, high-quality results in desalting, buffer exchange, and biomolecule purification workflows. The matrix handles pH and solvent extremes with ease—making it a robust choice for research and production chromatography where reproducibility and gentle separation are paramount.
