Description
SMART Chromatography™ Column for His-tag Purification Using Ni-NTA Resin
SMART Chromatography™ Ni-NTA from emp BIOTECH is a ready-to-use column packed with ZetaCell Ni-NTA resin—an agarose-based affinity medium functionalized with immobilized nickel-chelated nitrilotriacetic acid (Ni²⁺-NTA). Designed specifically for SMART Chromatography™, this column enables efficient purification of His-tagged recombinant proteins directly from cell-containing feedstreams without the need for clarification.
The large, highly cross-linked agarose beads (250 µm) provide unhindered flow of particulates while maintaining excellent mechanical strength and low backpressure. Binding is specific and robust, allowing elution under native or denaturing conditions with imidazole, histidine, or low-pH buffers. Its compatibility with SMART Radial Flow Chromatography supports linear scalability from development to manufacturing.
Performance Highlights
- ≥ 40 mg/mL binding capacity for His-tagged proteins
- ≥ 20 µmol Ni²⁺ per mL resin
- Designed for non-clarified feeds
- Flow rate ≥ 500 cm/h, pressure stable up to 3 bar
- Suitable for native or denaturing elution conditions
- Hydrophilic, cross-linked 250 µm agarose matrix
Specifications
- Resin: ZetaCell Ni-NTA
- Matrix: Highly cross-linked agarose
- Ligand: Ni²⁺-charged nitrilotriacetic acid (NTA)
- Bead Diameter: Average 250 µm
- Application: IMAC (Affinity Chromatography)
- Storage Buffer: 20% ethanol
Applications
- Purification of His-tagged recombinant proteins
- IMAC workflows with SMART Chromatography™
- Direct capture from non-clarified cell lysates
- Native or denaturing protein purification protocols
Why Choose SMART Chromatography™ Ni-NTA?
SMART Chromatography™ Ni-NTA columns combine the efficiency of immobilized metal affinity purification with the process simplicity of SMART technology. The large bead geometry and low backpressure make them ideal for processing viscous or particle-rich feeds. With strong affinity for histidine-tagged proteins and compatibility with linear scale-up, SC-4429 provides an excellent tool for reproducible, high-yield protein capture in bioprocessing environments.
