Description
SMART Chromatography™ Column for His-tag Purification Using Metal-Free NTA Resin
SMART Chromatography™ NTA (metal-free) from emp BIOTECH is a ready-to-use column packed with ZetaCell NTA resin—a hydrophilic, cross-linked agarose medium functionalized with uncharged nitrilotriacetic acid (NTA). Designed for use with SMART Chromatography™, the column supports purification of His-tagged recombinant proteins when charged with divalent metal ions such as Ni²⁺, Co²⁺, Cu²⁺, or Zn²⁺, offering flexible metal coordination based on protein-specific binding preferences.
The 250 µm agarose beads provide a low-backpressure, high-flow matrix optimized for direct processing of non-clarified cell lysates. Proteins are captured via selective affinity to chelated metals and eluted under native or denaturing conditions using imidazole, histidine, or low-pH buffers. The column supports linear scalability from lab-scale testing to full manufacturing using SMART Radial Flow Chromatography.
Performance Highlights
- Supports flexible chelation with Ni²⁺, Co²⁺, Cu²⁺, or Zn²⁺
- ≥ 40 mg/mL binding capacity for His-tagged proteins (Ni-charged)
- ≥ 20 µmol chelation capacity per mL resin
- Designed for particle-rich and non-clarified feedstreams
- Linear flow rate ≥ 500 cm/h, pressure stability up to 3 bar
- Hydrophilic, cross-linked 250 µm agarose beads
Specifications
- Resin: ZetaCell NTA (metal-free)
- Matrix: Highly cross-linked agarose
- Ligand: Nitrilotriacetic acid (uncharged)
- Bead Diameter: Average 250 µm
- Application: IMAC (Affinity Chromatography)
- Storage Buffer: 20% ethanol
Applications
- His-tagged protein purification using metal-charged NTA
- IMAC workflows for flexible metal coordination
- Native or denaturing protein purification
- Direct capture from non-clarified lysates
- High-throughput and scalable SMART Chromatography™
Why Choose SMART Chromatography™ NTA (metal-free)?
SMART Chromatography™ NTA (metal-free) gives you the flexibility to choose your metal ion—tailoring selectivity and elution conditions to your target protein. Combined with the robustness of SMART technology, this column enables reproducible, high-yield purification directly from crude samples, offering an ideal solution for method development, scale-up, and production environments where flexibility and flow performance matter.
