Description
SMART Chromatography™ Antibody Capture with NaOH-Stable Protein A Resin
SMART Chromatography™ Protein A Elevate from emp BIOTECH is a pre-packed FPLC column containing large-diameter beaded agarose resin surface-modified with a NaOH-tolerant recombinant Protein A ligand. Designed for direct purification of antibodies from cell-containing feedstocks, this resin combines robust base stability with uncluttered flow dynamics in SMART™ columns.
The 250 µm cross-linked agarose beads withstand system pressures up to 3 bar and allow cleaning-in-place (CIP) with 1 M NaOH for 15 minutes, maintaining consistent antibody binding capacity across multiple cycles. This makes the column particularly suited for continuous workflows in biomanufacturing environments.
Performance Highlights
- ≥ 30 mg/mL binding capacity (human IgG)
- ≥ 80% particles in the 140–360 µm range
- Linear velocity ≥ 300 cm/h
- Stable in 1 M NaOH for ≥ 15 minutes
- Operates efficiently at up to 3 bar system pressure
- Compatible with clarified and unclarified antibody feedstreams
Specifications
- Matrix: Highly cross-linked beaded agarose
- Ligand: NaOH-tolerant recombinant Protein A
- Bead Diameter: Average 250 µm
- Column Format: Pre-packed SMART™ FPLC column
Applications
- Antibody purification directly from cell culture
- Immunoglobulin G (IgG) and monoclonal antibody capture
- Continuous processing and perfusion bioprocessing
- NaOH-compatible CIP protocols
Why Choose SMART Chromatography™ Protein A Elevate?
This SMART Chromatography™ column leverages base-stable Protein A chemistry with clog-resistant bead design, enabling robust antibody capture even from unclarified feedstocks. Its proven alkaline durability and reproducible performance simplify purification workflows and extend column lifetime, supporting high-throughput, scalable bioprocesses.
