Description
Efficient Weak Anion Exchange in a Prepacked SMART™ Format
SMART Chromatography™ DEAE Boost, prepacked with ZetaCell DEAE Boost resin, is engineered for high-throughput weak anion exchange chromatography directly from cell-containing feed streams. The column is filled with large, highly cross-linked agarose beads derivatized with diethylaminoethyl (DEAE) ligands that offer enhanced surface-accessible binding sites for improved capacity. Its proprietary SMART™ design enables linear scalability, reduced backpressure, and efficient handling of crude bioreactor material.
The 250 µm bead geometry minimizes clogging and supports direct processing of unclarified samples while maintaining excellent resolution and reproducibility. The column is ideal for process development, scale-up validation, or production workflows requiring robust, simplified IEX purification.
Performance Highlights
- ≥ 80% of beads in the 140–360 µm range
- Linear velocity ≥ 2000 cm/h
- Operates efficiently at ≤ 3 bar system pressure
- Ionic capacity: 0.08–0.16 mmol Cl⁻/mL
- Compatible with clarified and unclarified feeds
- Packed and ready-to-use for SMART™ Chromatography
Specifications
- Resin: ZetaCell DEAE Boost
- Matrix: Cross-linked agarose, 250 µm average bead diameter
- Functional Group: Diethylaminoethyl (DEAE), weak anion exchanger
- pH Stability: 2–14 (short term), 3–12 (long term)
Applications
- Weak anion exchange (AEX) chromatography
- Capture and polishing of negatively charged biomolecules
- Bioprocessing directly from crude lysates or cell cultures
- Linear scale-up for continuous or batch production
Why Choose SMART™ DEAE Boost?
SMART Chromatography™ DEAE Boost simplifies downstream processing by combining scalable packed-bed technology with DEAE Boost’s enhanced ligand accessibility. Designed for challenging feedstocks, it enables efficient IEX purification with reduced pre-processing, high flow tolerance, and robust binding performance—delivering speed, capacity, and consistency in a ready-to-use format.
