Description
SMART Chromatography™ DEAE Column — Ion Exchange Separation for Cell-Containing Feeds
The SMART Chromatography™ DEAE column from emp BIOTECH is pre-packed with ZetaCell DEAE, a large-bead cross-linked agarose resin functionalized with diethylaminoethyl (DEAE), a weak anion exchange ligand. Specially engineered for direct processing of cell-containing feeds, this column enables efficient capture of negatively charged biomolecules without prior clarification steps. Its design supports high flow rates and low backpressure, making it ideal for scale-up and continuous downstream processing.
The resin’s weak anion exchange capacity allows flexible separation of a broad range of biomolecules under mild pH conditions. SMART Chromatography™ combines radial flow geometry with surface-accessible ligand distribution to increase throughput, reduce fouling, and simplify integration into purification workflows.
Performance Highlights
- Surface-accessible DEAE ligands for enhanced capacity
- Weak anion exchange functionality for flexible biomolecule capture
- Optimized for flow-through of cell-containing feed streams
- High linear flow rate (≥ 2000 cm/h) with low backpressure
- Operates up to 3 bar
- Resin: ZetaCell DEAE with 250 µm bead diameter
Specifications
- Matrix: Cross-linked beaded agarose
- Ligand: Diethylaminoethyl (DEAE)
- Bead Diameter: Average 250 µm
- Storage: 2–30 °C in 20% ethanol
- Column Format: SMART™ column, pre-packed and ready-to-use
Applications
- Ion exchange chromatography (AEX)
- Purification of negatively charged proteins and nucleic acids
- Direct processing of cell-containing harvests
- Continuous or perfusion-based downstream workflows
Why Choose the SMART™ DEAE Column?
This pre-packed SMART™ column simplifies process development and scale-up for weak anion exchange. With DEAE-functionalized ZetaCell resin and clog-resistant bead geometry, it supports direct loading of unclarified harvests, reducing process steps and increasing operational efficiency. It’s a robust solution for scalable, high-throughput purification.
