Description
SMART Chromatography™ Column with Strong Anion Exchange Resin for Clarified and Cell-Containing Feeds
SMART Chromatography™ Q, pre-packed with ZetaCell Q resin, is a high-performance strong anion exchange column designed for direct capture and purification of negatively charged biomolecules. Based on highly cross-linked agarose beads derivatized with quaternary ammonium ligands, this column is tailored for SMART Chromatography™ applications, enabling efficient processing of clarified and unclarified feed streams.
The large 250 µm beads promote unobstructed flow, minimizing clogging even in the presence of cell debris, and support rapid separation with high binding capacity and consistent performance under industrial process conditions.
Performance Highlights
- ≥ 80% beads in the 140–360 µm range (average 250 µm)
- Ionic capacity: 0.08–0.16 mmol Cl⁻/mL
- Supports linear velocities ≥ 2000 cm/h
- Operates at up to 3 bar system pressure
- Stable from pH 2–14 (short term), 3–12 (long term)
- Compatible with SMART Chromatography™ systems
Specifications
- Matrix: Cross-linked beaded agarose
- Ligand: Quaternary ammonium (Q), strong anion exchanger
- Average Bead Diameter: 250 µm
- Resin: ZetaCell Q
- Storage Buffer: 20% ethanol
Applications
- SMART Chromatography™ of negatively charged proteins and biomolecules
- High-throughput anion exchange from clarified or unclarified feeds
- Bioprocessing workflows requiring scale-up and robust flow properties
- Primary capture or polishing of acidic proteins, DNA, or virus particles
Why Choose SMART Chromatography™ Q?
SMART Chromatography™ Q combines high binding capacity with the clog-resistant geometry of ZetaCell resin, enabling seamless integration into bioprocess workflows with cell-containing or clarified feeds. The strong anion exchange functionality ensures reliable purification under harsh conditions, while the pre-packed column design simplifies process setup and scale-up.
