Description
Streamlined SMART™ Ion Exchange with CM Functionalization
SMART Chromatography™ CM from emp BIOTECH is a ready-to-use column packed with large cross-linked agarose beads functionalized with a carboxymethyl (CM) ligand for weak cation exchange chromatography. Designed for SMART™ workflows, the column enables direct processing of cell-containing feedstocks without prior clarification, supporting efficient separation of positively charged biomolecules under mild conditions. The surface-accessible ligands and large-pore geometry ensure high throughput and reduced risk of clogging, even in unclarified feeds.
Performance Highlights
- Packed with ZetaCell CM weak cation exchange resin
- Mean particle size: 250 µm, cross-linked agarose beads
- Surface-accessible ligands for efficient interaction
- High flow rates with low back pressure
- Operates at pressures up to 3 bar
- Compatible with mammalian, yeast, and bacterial cultures
Specifications
- Resin: ZetaCell CM
- Ligand: Carboxymethyl (CM), weak cation exchanger
- Base Matrix: Cross-linked agarose
- Bead Size: 250 µm (average)
Applications
- Capture and intermediate purification of positively charged biomolecules
- Cation exchange workflows under mild conditions
- Continuous or perfusion-based bioprocessing
- Direct processing of cell-containing feeds
Why Choose SMART Chromatography™ CM?
SMART Chromatography™ CM allows purification directly from non-clarified feedstreams, eliminating the need for upstream centrifugation or filtration. This results in shorter process times, reduced system complexity, and lower operational costs—while maintaining robust performance and consistent resolution across scales.
