Description
Strong Cation Exchange for High-Throughput Bioprocessing in SMART™ Columns
SMART Chromatography™ SP Boost from emp BIOTECH is a pre-packed FPLC column designed for efficient ion exchange purification using SMART Chromatography™. The column is filled with ZetaCell SP Boost resin—large, highly cross-linked agarose beads functionalized with sulphopropyl (SP), a strong cation exchange ligand. With surface-localized ion exchange sites, this resin delivers enhanced binding capacity, low backpressure, and clog-resistant flow—ideal for cell-containing feedstreams.
The robust design supports high linear flow rates and alkaline stability, enabling fast, reproducible separation of positively charged biomolecules across a wide pH range. Pre-packed in durable PMMA housing with stainless steel frits and M6 connectors, the column is ready to integrate into any FPLC workflow.
Performance Highlights
- Optimized for SMART Chromatography™ of proteins and biomolecules
- ≥ 80% of particles in the 140–360 µm range
- Linear velocity ≥ 1000 cm/h (15 mL/min)
- Ion exchange capacity: 0.08–0.16 mmol H⁺/mL
- Operates up to 3 bar pressure
- pH stability: 2–14 (short term), 4–12 (long term)
- Packed in 0.2 M sodium acetate, 20% ethanol buffer
Specifications
- Resin: ZetaCell SP Boost (sulphopropyl ligand)
- Bead Diameter: ~250 µm
- Column Material: PMMA with stainless steel frits
- Format: FPLC column (5 or 10 mL versions)
Applications
- Cation exchange chromatography (CEX)
- Purification of basic proteins and biomolecules
- Processing of cell-containing feedstocks
- Scalable, robust downstream purification
Why Choose SMART Chromatography™ SP Boost?
SMART Chromatography™ SP Boost combines high capacity with pressure stability, resin longevity, and compatibility with unclarified feed streams. Designed for bioprocessing environments that demand speed and reproducibility, this column simplifies cation exchange workflows and supports continuous or batch purification strategies with ease.
