Description
TurboTag 557 Protein Labeling Kit for High-Performance TAMRA Conjugation
TurboTag 557 from emp BIOTECH is a complete protein labeling and purification kit for covalent conjugation with carboxy-tetramethylrhodamine (TAMRA), a bright and stable red fluorophore. Designed for proteins ≥ 25 kDa—especially antibodies—the kit uses a highly reactive NHS ester to form stable amide linkages between TAMRA and primary amines on the target protein.
The resulting protein-dye conjugates exhibit strong fluorescence with excitation at 554 nm and emission at 577 nm, ideal for red-channel fluorescence applications in imaging, detection, and analysis.
Each kit includes 50 nmol of reactive dye—sufficient to label up to 15 nmol of protein—and is compatible with optimized protocols for labeling 1 mg or 100 µg of IgG. TurboTag 557 provides a simple, reproducible workflow for generating high-quality, fluorescently labeled proteins.
Performance Highlights
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Efficient labeling of proteins ≥ 25 kDa, especially antibodies
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Excitation/emission at 554/577 nm (TAMRA) for red-channel detection
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Stable amide bond formation via NHS ester chemistry
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Optimized for IgG labeling (1 mg or 100 µg protocols available)
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High signal intensity with low background interference
Properties
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Molecular Weight: 527.5
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Empirical Formula: C₂₉H₂₅N₃O₇
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Absorption [λmax]: 554 ± 3 nm
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Emission [λmax]: 577 ± 3 nm
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Extinction Coefficient (ε): 80,000 M⁻¹·cm⁻¹
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Reactive Moiety: NHS ester (reacts with 1° amines to form stable amide linkage)
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Storage: −20 °C, dry, protected from light
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Labeling Capacity: Up to 15 nmol protein per 50 nmol dye vial
Applications
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Fluorescent labeling of IgG and other proteins
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Protein tracking in red-fluorescence detection platforms
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Quantitative and qualitative protein detection
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Fluorescence-based diagnostics and imaging workflows
Why Choose TurboTag™ 557?
TurboTag 557 offers bright and reliable red fluorescence through conjugation with TAMRA, a trusted fluorophore for protein labeling. It supports robust covalent coupling via NHS ester chemistry, delivering consistent labeling results across diverse proteins—especially antibodies. With its strong fluorescence signal and compatibility with optimized IgG protocols, TurboTag 557 enables fast and reproducible labeling for red-channel imaging, detection, and protein-based diagnostics.
