Description
TurboTag 587 Protein Labeling Kit for Robust X-Rhodamine Conjugation
TurboTag 587 from emp BIOTECH is a complete labeling and purification kit designed for covalent conjugation of proteins with X-Rhodamine (ROX). Ideal for proteins ≥ 25 kDa, particularly antibodies, the kit utilizes a highly reactive NHS ester of carboxy-X-rhodamine to form stable amide linkages with primary amines. The resulting conjugates exhibit strong fluorescence with excitation at 587 nm and emission at 599 nm, making this kit especially suited for red-channel detection in fluorescence-based assays.
Each vial contains 50 nmol of reactive TurboTag 587 dye—sufficient to label up to 15 nmol of protein. For optimal performance, follow the included protocols for labeling 1 mg or 100 µg of IgG.
Performance Highlights
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High-efficiency labeling of proteins ≥ 25 kDa, including IgG antibodies
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Bright red fluorescence with excitation/emission at 587/599 nm
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Covalent NHS ester chemistry for stable, amide-linked conjugates
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Pre-validated protocols for 1 mg and 100 µg IgG
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Easy-to-use kit format for consistent, high-yield labeling
Properties
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Molecular Weight: 631.7
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Empirical Formula: C₃₇H₃₃N₃O₇
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Absorption [λmax]: 587 ± 3 nm
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Emission [λmax]: 599 ± 3 nm
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Extinction Coefficient (ε): 95,000 M⁻¹·cm⁻¹
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Reactive Moiety: NHS ester (reacts with primary amines to form stable amide linkage)
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Storage: −20 °C, dry, protected from light
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Labeling Capacity: Up to 15 nmol protein per 50 nmol dye vial
Applications
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Fluorescent labeling of antibodies and other proteins
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Red-channel detection in fluorescence assays
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Multiplex imaging and flow cytometry
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Fluorescence-based diagnostics and biomarker tracking
Why Choose TurboTag™ 587?
TurboTag 587 offers reliable and intense fluorescent labeling using the well-characterized X-Rhodamine (ROX) dye, ideal for high-sensitivity red-fluorescence detection. The NHS ester chemistry ensures efficient and stable covalent bonding, while the optimized kit format supports rapid, reproducible labeling workflows. TurboTag 587 is the trusted solution for researchers seeking consistent red-fluorescent conjugates for demanding proteomics and immunoassay applications.
