Description
TurboTag 498 Protein Labeling Kit for Bright Fluorescein (FAM) Conjugation
TurboTag 498 from emp BIOTECH is a complete labeling and purification kit for covalent conjugation of proteins with fluorescein (FAM). Designed for high-efficiency labeling of proteins ≥ 25 kDa—especially antibodies—this kit utilizes a reactive NHS ester of carboxy-fluorescein to generate bright and stable fluorescent conjugates.
The resulting protein-dye conjugates exhibit strong fluorescence with excitation at 498 nm and emission at 522 nm, enabling sensitive detection in fluorescence microscopy, flow cytometry, and immunoassay workflows. Each 50 nmol vial of reactive dye can label up to 15 nmol of protein, and optimized protocols are provided for 1 mg or 100 µg of IgG.
Performance Highlights
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Efficient fluorescent labeling of antibodies and other proteins ≥ 25 kDa
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Bright fluorescein signal with excitation/emission at 498/522 nm
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Stable covalent linkage via NHS ester chemistry
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Suitable for monoclonal and polyclonal IgG using ready-to-run protocols
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Pre-packed kit format for streamlined conjugation and purification
Properties
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Molecular Weight: 473.0
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Empirical Formula: C₂₅H₁₅NO₉
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Absorption [λmax]: 498 ± 3 nm
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Emission [λmax]: 522 ± 3 nm
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Extinction Coefficient (ε): 70,000 M⁻¹·cm⁻¹
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Reactive Moiety: NHS ester (reacts with primary amines to form stable amide linkage)
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Storage: −20 °C, dry, protected from light
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Labeling Capacity: Up to 15 nmol of protein per 50 nmol dye vial
Applications
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Fluorescent protein labeling for imaging and analysis
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Immunoassays and diagnostic detection
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Antibody tracking and signal enhancement
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Quantitative fluorescence-based studies
Why Choose TurboTag™ 498?
TurboTag 498 enables efficient and reproducible labeling of proteins with fluorescein for a wide range of fluorescence-based applications. Its NHS ester chemistry guarantees strong, covalent attachment under mild conditions, while its high extinction coefficient supports bright, stable fluorescence. The kit’s streamlined protocol and high labeling capacity make it ideal for researchers seeking fast, reliable conjugation of antibodies or other amine-containing proteins with minimal hands-on time.
