Description
TurboTag 667 Protein Labeling Kit for Redox and Electrochemical Protein Conjugation
TurboTag 667 from emp BIOTECH is a protein labeling and purification kit that utilizes a dicarboxy methylene blue NHS ester for covalent conjugation of proteins ≥ 25 kDa. Ideal for antibodies and other large biomolecules, this kit forms stable amide linkages through NHS ester chemistry. While the conjugates exhibit very weak fluorescence(excitation/emission ~665/670 nm), methylene blue is widely used in redox reactions, nucleic acid intercalation, and as an electroactive label in biosensing applications.
Each vial provides 50 nmol of reactive dye, enabling labeling of up to 15 nmol of protein. The kit includes protocols optimized for labeling both 1 mg and 100 µg of IgG, facilitating reproducible conjugation and minimal hands-on time.
Performance Highlights
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Covalent protein labeling with dicarboxy methylene blue for redox and electrochemical workflows
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NHS ester chemistry ensures stable amide bond formation with primary amines
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Weak fluorescence makes it ideal for use as a redox dye or electrochemical reporter
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Compatible with standard labeling protocols for IgG (1 mg and 100 µg)
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Suitable for nucleic acid intercalation and electron transfer-based biosensors
Properties
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Molecular Weight: 439.5
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Empirical Formula: C₂₂H₂₃N₄O₈SCl
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Absorption [λmax]: 665 ± 3 nm
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Emission [λmax]: 670 ± 3 nm (very weak fluorescence)
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Extinction Coefficient (ε): 81,000 M⁻¹·cm⁻¹
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Reactive Moiety: NHS ester (reacts with primary amines to form stable amide linkage)
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Storage: −20 °C, dry, protected from light
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Labeling Capacity: Up to 15 nmol protein per 50 nmol dye vial
Applications
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Redox labeling of antibodies and proteins
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Electrochemical biosensing
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Protein labeling for electron transfer studies
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Nucleic acid intercalation and visualization
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Weak fluorescence applications and experimental tracking
Why Choose TurboTag™ 667?
TurboTag 667 provides a specialized protein labeling option for researchers working in redox chemistry, electrochemical detection, or nucleic acid research. Unlike conventional fluorescent labels, methylene blue offers unique electroactive and intercalating properties, allowing detection beyond standard fluorescence-based methods. With robust NHS ester chemistry, optimized IgG labeling protocols, and compatibility with a wide range of protein-based assays, TurboTag 667 is a reliable choice for redox-based bioconjugation workflows.
