Description
TurboTag 750 Protein Labeling Kit for Near-Infrared DY-750 Conjugation
TurboTag 750 from emp BIOTECH is a complete protein labeling and purification kit optimized for the covalent conjugation of proteins using DY-750, a high-performance near-infrared fluorophore. This kit is ideal for labeling proteins ≥ 25 kDa, particularly antibodies, with a reactive NHS ester of DY-750 that forms stable amide linkages with primary amines. The resulting conjugates exhibit excitation/emission maxima at 747 nm and 776 nm, offering deep-tissue imaging capabilities and minimal background interference.
Each vial contains 50 nmol of reactive dye, sufficient to label up to 15 nmol of protein. TurboTag 750 includes protocols optimized for both 1 mg and 100 µg IgG samples, ensuring rapid and reproducible labeling for diagnostic or research applications requiring high sensitivity.
Performance Highlights
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Efficient labeling of proteins ≥ 25 kDa, including IgG and monoclonal antibodies
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Strong near-infrared fluorescence (excitation/emission at 747/776 nm)
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NHS ester chemistry enables stable, covalent labeling via primary amines
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Compatible with pre-validated protocols for 1 mg and 100 µg IgG
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Ideal for deep-tissue imaging, low-background detection, and multiplex assays
Properties
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Molecular Weight: 810.03
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Empirical Formula: C₄₆H₅₅N₃O₈S
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Absorption [λmax]: 747 ± 3 nm
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Emission [λmax]: 776 ± 3 nm
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Extinction Coefficient (ε): 270,000 M⁻¹·cm⁻¹
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Reactive Moiety: NHS ester (reacts with 1° amines to form stable amide linkage)
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Storage: −20 °C, dry, protected from light
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Labeling Capacity: Up to 15 nmol protein per 50 nmol dye vial
Applications
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Fluorescent labeling of proteins and antibodies
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In vivo imaging and near-infrared detection
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Immunoassays and signal amplification
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Multiplex fluorescence analysis
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Deep-tissue or low-autofluorescence environments
Why Choose TurboTag™ 750?
TurboTag 750 delivers superior fluorescent labeling with DY-750, combining high quantum yield, near-infrared spectral properties, and robust NHS ester chemistry for stable conjugation. The kit supports high-sensitivity detection and low-background imaging, making it ideal for demanding diagnostic workflows, multiplex assays, and in vivo studies. Its pre-validated protocols ensure consistent, reproducible results in under an hour.
