Description
TurboTag 594 Protein Labeling Kit for Bright DY-594 Fluorescent Conjugation
TurboTag 594 from emp BIOTECH is a complete protein labeling and purification kit based on DYOMICS’ DY-594dye. Optimized for proteins ≥ 25 kDa, especially antibodies, this kit uses a highly reactive NHS ester to form covalent amide bonds with primary amines. The resulting protein-dye conjugates emit a strong fluorescent signal, with excitation and emission maxima at 594 nm and 615 nm, making it ideal for red-channel applications such as flow cytometry, fluorescence microscopy, and immunoassays.
Each vial delivers 50 nmol of reactive dye—sufficient to label up to 15 nmol of protein. The kit includes optimized protocols for labeling both 1 mg and 100 µg of IgG, ensuring reproducibility and high yield.
Performance Highlights
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Efficient NHS-ester-based labeling of proteins ≥ 25 kDa
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Strong red fluorescence (λ_ex: 594 nm / λ_em: 615 nm)
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Stable amide linkage ensures long-term conjugate stability
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Pre-validated protocols for 1 mg and 100 µg IgG
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Streamlined workflow with integrated purification steps
Properties
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Molecular Weight: 1078.1
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Empirical Formula: C₄₃H₄₅N₅O₁₇S₄Na₂
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Absorption [λmax]: 594 ± 3 nm
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Emission [λmax]: 615 ± 3 nm
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Extinction Coefficient (ε): 92,000 M⁻¹·cm⁻¹
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Reactive Moiety: NHS ester (reacts with primary amines to form stable amide linkage)
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Storage: −20 °C, dry, protected from light
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Labeling Capacity: Up to 15 nmol protein per 50 nmol dye vial
Applications
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Fluorescent labeling of proteins and antibodies
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Red-channel detection in immunoassays and western blots
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Fluorescence microscopy and multiplex imaging
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Biomarker detection and high-sensitivity diagnostics
Why Choose TurboTag™ 594?
TurboTag 594 offers bright red fluorescence, robust NHS ester reactivity, and an optimized labeling protocol in a single convenient kit. Its DY-594 dye enables reliable detection of protein targets in a wide range of fluorescence-based techniques. With high yield, low background, and covalent stability, TurboTag 594 is a dependable choice for researchers needing high-performance labeling for proteins ≥ 25 kDa.
