Description
TurboTag 565 Protein Labeling Kit for High-Sensitivity DY-555 Conjugation
TurboTag 565 from emp BIOTECH is a complete protein labeling and purification kit designed for covalent conjugation of proteins with DY-555, a high-brightness red fluorophore. Using a reactive NHS ester of DYOMICS’ DY-555 dye, the kit enables stable amide bond formation with primary amines on proteins ≥ 25 kDa—particularly antibodies.
The resulting protein-dye conjugates offer intense fluorescence with excitation at 547 nm and emission at 572 nm, making TurboTag 565 ideal for red-channel fluorescence applications in imaging, flow cytometry, and immunoassays.
Each kit contains 50 nmol of reactive dye, suitable for labeling up to 15 nmol of protein, and includes optimized protocols for 1 mg and 100 µg IgG labeling to ensure consistent, high-quality results.
Performance Highlights
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Fast, efficient covalent labeling of proteins ≥ 25 kDa
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Bright red fluorescence with excitation/emission at 547/572 nm
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NHS ester chemistry ensures stable amide bond formation
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Compatible with standard protocols for IgG labeling
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Reliable results for fluorescence detection, imaging, and diagnostics
Properties
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Molecular Weight: 733.27
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Empirical Formula: C₃₉H₄₅N₄O₈Cl
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Absorption [λmax]: 547 ± 3 nm
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Emission [λmax]: 572 ± 3 nm
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Extinction Coefficient (ε): 100,000 M⁻¹·cm⁻¹
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Reactive Moiety: NHS ester (reacts with 1° amines to form stable amide linkage)
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Storage: −20 °C, dry, protected from light
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Labeling Capacity: Up to 15 nmol protein per 50 nmol dye vial
Applications
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Fluorescent labeling of antibodies and proteins
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Protein detection in fluorescence assays and blotting
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Flow cytometry and fluorescence microscopy
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Diagnostic assay development and red-channel multiplexing
Why Choose TurboTag™ 565?
TurboTag 565 enables high-efficiency fluorescent labeling with DY-555, a red-emitting dye optimized for high signal intensity and low background. Its NHS ester chemistry ensures stable conjugation to primary amines, making it ideal for antibody labeling workflows. With strong excitation/emission performance and compatibility with standard IgG protocols, TurboTag 565 delivers consistent, sensitive detection for demanding protein analysis and diagnostics.
