Description
TurboTag 530 Protein Labeling Kit for High-Performance DY-530 Conjugation
TurboTag 530 from emp BIOTECH is a complete protein labeling and purification kit based on DYOMICS’ DY-530 fluorophore. Designed for covalent conjugation of proteins ≥ 25 kDa—especially antibodies—this kit uses a reactive NHS ester derivative of DY-530 to create bright, stable dye-protein conjugates. The resulting labeled proteins exhibit fluorescence excitation and emission at 539 nm and 561 nm, respectively, making them ideal for fluorescence-based assays, imaging, and diagnostics.
Each 50 nmol vial of TurboTag 530 dye labels up to 15 nmol of protein. The kit is optimized for high-yield, covalent amide bond formation and includes protocols for labeling both 1 mg and 100 µg of IgG. With straightforward workflows and reliable results, TurboTag 530 is suitable for both routine and demanding fluorescence applications.
Performance Highlights
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Efficient covalent labeling of proteins ≥ 25 kDa (including antibodies)
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Bright fluorescence with DY-530 (excitation/emission at 539/561 nm)
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Stable conjugates formed via NHS ester–primary amine reaction
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Pre-validated protocols for 1 mg and 100 µg IgG
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Convenient kit format includes dye and purification materials
Properties
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Molecular Weight: 792.82
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Empirical Formula: C₃₅H₃₇N₄O₁₂S₂Na
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Absorption [λmax]: 539 ± 3 nm
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Emission [λmax]: 561 ± 3 nm
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Extinction Coefficient (ε): 100,000 M⁻¹·cm⁻¹
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Reactive Moiety: NHS ester (reacts with primary amines to form a stable amide linkage)
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Storage: −20 °C, dry, protected from light
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Labeling Capacity: Up to 15 nmol of protein per 50 nmol dye vial
Applications
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Fluorescent labeling of proteins and antibodies
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Immunoassays and molecular detection
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Imaging and microscopy workflows
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Fluorescence-based biomarker studies
Why Choose TurboTag™ 530?
TurboTag 530 offers a powerful solution for fast, high-yield fluorescent labeling of proteins using DY-530 dye. With robust NHS chemistry and reliable excitation/emission at 539/561 nm, this kit enables sensitive detection across a wide range of applications. Its compatibility with optimized protocols ensures consistent results with minimal hands-on time, supporting efficient conjugation of proteins for advanced fluorescence workflows.
