Description
TurboTag 777 Protein Labeling Kit for High-Sensitivity DY-777 Conjugation
TurboTag 777 from emp BIOTECH is a ready-to-use protein labeling and purification kit optimized for far-red/near-infrared (NIR) fluorescence applications. Using a highly reactive NHS ester derivative of DY-777, the kit covalently labels proteins ≥ 25 kDa—particularly antibodies—through stable amide bond formation. The resulting conjugates exhibit intense fluorescence with excitation/emission maxima at 770 nm and 788 nm, ideal for deep-tissue imaging, low-background detection, and multiplexed assays.
Each 50 nmol vial of reactive dye labels up to 15 nmol of protein. TurboTag 777 includes pre-validated protocols for both 1 mg and 100 µg IgG labeling, making it ideal for rapid adoption in research and diagnostic workflows.
Performance Highlights
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Optimized for labeling proteins ≥ 25 kDa, including monoclonal antibodies
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Strong near-infrared fluorescence at 770 nm (excitation) / 788 nm (emission)
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NHS ester chemistry enables stable covalent conjugation
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High molar extinction coefficient (ε = 240,000 M⁻¹·cm⁻¹) for sensitive detection
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Pre-optimized IgG labeling protocols for 1 mg and 100 µg samples
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Suitable for in vivo imaging, diagnostics, and multiplex detection
Properties
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Molecular Weight: 1048.14
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Empirical Formula: C₄₉H₅₁N₃O₁₄S₃Na₂
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Absorption [λmax]: 770 ± 3 nm
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Emission [λmax]: 788 ± 3 nm
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Extinction Coefficient (ε): 240,000 M⁻¹·cm⁻¹
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Reactive Moiety: NHS ester (reacts with primary amines to form stable amide linkage)
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Storage: −20 °C, dry, protected from light
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Labeling Capacity: Up to 15 nmol protein per 50 nmol dye vial
Applications
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Fluorescent labeling of proteins and antibodies
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In vivo imaging and near-infrared fluorescence assays
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Low-background detection in diagnostic workflows
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Multiplex fluorescence-based detection systems
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Protein tracking and biodistribution studies
Why Choose TurboTag™ 777?
TurboTag 777 combines the brightness and stability of the DY-777 fluorophore with reliable NHS ester reactivity to deliver superior labeling results. Ideal for demanding near-infrared applications, this kit ensures reproducibility, high sensitivity, and minimal background—making it a trusted choice for advanced imaging, diagnostics, and research involving large biomolecules.
