Description
TurboTag 752 Protein Labeling Kit for High-Sensitivity DY-752 Conjugation
TurboTag 752 from emp BIOTECH is a robust labeling and purification kit designed for covalent conjugation of proteins with the near-infrared fluorophore DY-752. Specifically engineered for proteins ≥ 25 kDa—including antibodies—TurboTag 752 enables the formation of highly stable amide-linked conjugates using a reactive NHS ester dye. The resulting conjugates exhibit strong near-IR fluorescence with excitation/emission maxima at 748 nm and 772 nm, enabling deep-tissue imaging, biodistribution studies, and multiplex fluorescence detection.
Each 50 nmol vial of reactive dye allows labeling of up to 15 nmol of protein. The kit is delivered with easy-to-follow protocols optimized for 1 mg and 100 µg IgG labeling.
Performance Highlights
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Tailored for proteins ≥ 25 kDa, including monoclonal antibodies
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Excitation/emission at 748/772 nm for high-sensitivity near-IR detection
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NHS ester chemistry ensures stable covalent conjugation
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High extinction coefficient (ε = 270,000 M⁻¹·cm⁻¹) for intense fluorescence
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Pre-optimized protocols for rapid and reproducible IgG labeling
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Compatible with multiplex assays and in vivo imaging
Properties
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Molecular Weight: 1028.15
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Empirical Formula: C₄₇H₅₅N₃O₁₄S₃Na₂
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Absorption [λmax]: 748 ± 3 nm
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Emission [λmax]: 772 ± 3 nm
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Extinction Coefficient (ε): 270,000 M⁻¹·cm⁻¹
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Reactive Moiety: NHS ester (reacts with primary amines to form a stable amide linkage)
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Storage: −20 °C, dry, protected from light
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Labeling Capacity: Up to 15 nmol protein per 50 nmol dye vial
Applications
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Fluorescent labeling of antibodies and proteins
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Near-infrared in vivo imaging
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Multiplex fluorescence detection
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Protein tracking and pharmacokinetics
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Low-background diagnostic assays
Why Choose TurboTag™ 752?
TurboTag 752 empowers researchers with an advanced labeling solution optimized for high-sensitivity near-infrared detection. Its robust NHS ester chemistry ensures stable conjugation, while the intense fluorescence of DY-752 supports imaging and analytical workflows with minimal interference. With optimized protocols for standard IgG quantities, TurboTag 752 enables fast, reproducible, and scalable protein-dye conjugation.
