Description
TurboTag 749 Protein Labeling Kit for High-Sensitivity DY-749P1 Conjugation
TurboTag 749 from emp BIOTECH is a complete protein labeling and purification kit engineered for covalent conjugation of proteins with the near-infrared fluorophore DY-749P1. The kit is optimized for labeling proteins ≥ 25 kDa, particularly antibodies, using a reactive NHS ester that forms stable amide linkages with primary amines. The resulting conjugates exhibit excitation/emission maxima at 758 nm and 779 nm, providing deep-tissue imaging capabilities and minimal background fluorescence.
Each kit includes 120 nmol of reactive dye, sufficient to label up to 15 nmol of protein. TurboTag 749 is supplied with protocols optimized for both 1 mg and 100 µg IgG, ensuring rapid and reproducible conjugation under mild reaction conditions.
Performance Highlights
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Efficient fluorescent labeling of proteins ≥ 25 kDa, especially IgG and monoclonal antibodies
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Bright near-infrared signal with DY-749P1 (excitation/emission at 758/779 nm)
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Covalent labeling via NHS ester chemistry ensures high conjugate stability
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Pre-validated labeling protocols for 1 mg or 100 µg IgG
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Ideal for low-background and deep-tissue detection workflows
Properties
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Molecular Weight: 1092.11
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Empirical Formula: C₄₄H₅₂N₃O₁₇S₄Na₃
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Absorption [λmax]: 759 ± 3 nm
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Emission [λmax]: 780 ± 3 nm
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Extinction Coefficient (ε): 240,000 M⁻¹·cm⁻¹
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Reactive Moiety: NHS ester (reacts with primary amines to form stable amide linkage)
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Storage: −20 °C, dry, protected from light
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Labeling Capacity: Up to 15 nmol protein per 120 nmol dye vial
Applications
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Fluorescent labeling of antibodies and high-molecular-weight proteins
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Near-infrared detection in immunoassays and diagnostics
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In vivo imaging and biodistribution studies
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Multiplex assays with minimal spectral overlap
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Sensitive biomarker tracking in complex environments
Why Choose TurboTag™ 749?
TurboTag 749 enables high-efficiency protein labeling with DY-749P1, a near-infrared fluorophore that combines high quantum yield with exceptional tissue penetration and low autofluorescence. Its NHS ester chemistry ensures covalent and stable dye-protein conjugation, while the included protocols streamline the workflow for IgG and other protein types. Whether you’re developing fluorescent diagnostics or running in vivo detection assays, TurboTag 749 offers robust, reproducible labeling with excellent optical performance.
