Description
TurboTag 701 Protein Labeling Kit for Near-Infrared DY-701 Conjugation
TurboTag 701 from emp BIOTECH is a complete fluorescent protein labeling and purification kit designed for the covalent conjugation of proteins with DY-701, a near-infrared dye. Ideal for proteins ≥ 25 kDa—including antibodies—the kit utilizes a reactive NHS ester of DY-701 that forms stable amide linkages with primary amines. The resulting protein-dye conjugates exhibit excitation/emission maxima at 706 nm and 735 nm, making them highly suitable for low-background fluorescence detection and deep-tissue imaging.
Each vial contains 50 nmol of reactive dye, sufficient to label up to 15 nmol of protein. The kit includes optimized protocols for both 1 mg and 100 µg IgG labeling, providing a robust, reproducible workflow for biochemical and diagnostic applications.
Performance Highlights
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Effective fluorescent labeling of proteins ≥ 25 kDa, especially IgG and other antibodies
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Near-infrared fluorescence with DY-701 (excitation/emission at 706/735 nm)
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NHS ester-mediated covalent labeling ensures stability and reproducibility
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Pre-validated protocols for labeling 1 mg or 100 µg of IgG
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Excellent performance for low-background and multiplex fluorescence assays
Properties
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Molecular Weight: 867.97
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Empirical Formula: C₄₃H₄₆N₃O₁₁S₃Na
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Absorption [λmax]: 706 ± 3 nm
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Emission [λmax]: 731 ± 3 nm
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Extinction Coefficient (ε): 140,000 M⁻¹·cm⁻¹
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Reactive Moiety: NHS ester (reacts with primary amines to form stable amide linkage)
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Storage: −20 °C, dry, protected from light
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Labeling Capacity: Up to 15 nmol protein per 50 nmol dye vial
Applications
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Fluorescent labeling of antibodies and proteins
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Near-infrared imaging and in vivo detection
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Immunoassays with low autofluorescence requirements
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Multiplexed biomarker detection in complex samples
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Fluorescent tracking in high-sensitivity protein analysis
Why Choose TurboTag™ 701?
TurboTag 701 enables fast, efficient labeling of proteins with the near-infrared dye DY-701 for demanding diagnostic and analytical applications. Its NHS ester chemistry ensures robust covalent conjugation, while its strong signal at 706/735 nm minimizes background noise in fluorescence-based assays. With optimized IgG protocols and excellent dye stability, TurboTag 701 delivers consistent results in multiplex imaging, biomarker detection, and protein tracking—even in complex biological environments.
