Description
TurboTag 677 Protein Labeling Kit for Far-Red DY-677 Conjugation
TurboTag 677 from emp BIOTECH is a high-performance labeling and purification kit for covalent conjugation of proteins with the far-red fluorophore DY-677. Designed for proteins ≥ 25 kDa—especially antibodies—this kit uses a highly reactive NHS ester derivative of DY-677 to ensure stable and efficient amide linkage formation. The resulting conjugates offer strong far-red fluorescence with excitation and emission maxima at 673 nm and 694 nm, respectively, ideal for advanced imaging and immunoassay applications.
Each kit contains 50 nmol of reactive dye, allowing labeling of up to 15 nmol of protein. Optimized IgG labeling protocols for both 1 mg and 100 µg are included to simplify workflows and ensure reproducibility.
Performance Highlights
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Effective covalent labeling of antibodies and other proteins ≥ 25 kDa
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Strong far-red signal with excitation/emission at 673/694 nm
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NHS ester-based chemistry for stable, high-yield conjugation
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Supports pre-optimized IgG labeling protocols (1 mg and 100 µg)
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Suitable for multiplex detection, in vivo imaging, and low-background assays
Properties
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Molecular Weight: 1022.09
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Empirical Formula: C₄₇H₄₉N₃O₁₄S₃Na₂
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Absorption [λmax]: 673 ± 3 nm
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Emission [λmax]: 694 ± 3 nm
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Extinction Coefficient (ε): 180,000 M⁻¹·cm⁻¹
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Reactive Moiety: NHS ester (forms stable amide linkages with primary amines)
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Storage: −20 °C, dry, protected from light
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Labeling Capacity: Up to 15 nmol of protein per 50 nmol dye vial
Applications
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Fluorescent labeling of antibodies and high-MW proteins
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Far-red detection in immunoassays and bioanalytical workflows
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In vivo and near-infrared (NIR) imaging
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Multiplex assays with minimal spectral overlap
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Protein tracking and biomarker discovery
Why Choose TurboTag™ 677?
TurboTag 677 delivers strong, stable far-red fluorescence through efficient conjugation of DY-677 dye to proteins. Its NHS ester chemistry guarantees reliable amide bond formation under mild conditions, making it ideal for sensitive applications like flow cytometry, NIR imaging, and immunodiagnostics. Optimized protocols streamline antibody labeling and ensure consistent, reproducible performance every time. For researchers seeking robust and high-sensitivity protein labeling in the far-red range, TurboTag 677 is a dependable solution.
