Description
TurboTag 652 Protein Labeling Kit for Near-Infrared DY-652 Conjugation
TurboTag 652 from emp BIOTECH is a complete labeling and purification kit designed for efficient covalent conjugation of proteins using DY-652, a near-infrared fluorophore. Ideal for proteins with molecular weights of ≥ 25 kDa, including antibodies, the kit utilizes a reactive NHS ester of DY-652 to form stable amide linkages with primary amines. The resulting conjugates exhibit strong fluorescence with excitation/emission maxima at 654 nm and 675 nm, ideal for high-sensitivity detection in diagnostic, imaging, and analytical applications.
Each vial contains 50 nmol of reactive dye, suitable for labeling up to 15 nmol of protein. The TurboTag 652 kit is supplied with pre-optimized protocols for labeling both 1 mg and 100 µg IgG, enabling fast and reproducible workflows with minimal hands-on time.
Performance Highlights
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Reliable fluorescent labeling of proteins ≥ 25 kDa, including IgG and monoclonal antibodies
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Strong near-infrared signal: excitation/emission at 654/675 nm
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NHS ester chemistry ensures robust covalent linkage for long-term conjugate stability
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Pre-validated protocols for 1 mg and 100 µg IgG labeling
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Ideal for low-background detection, multiplex fluorescence, and in vivo imaging
Properties
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Molecular Weight: 1002.11
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Empirical Formula: C₄₅H₅₃N₃O₁₄S₄Na₂
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Absorption [λmax]: 654 ± 3 nm
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Emission [λmax]: 675 ± 3 nm
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Extinction Coefficient (ε): 220,000 M⁻¹·cm⁻¹
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Reactive Moiety: NHS ester (reacts with primary amines to form stable amide linkage)
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Storage: –20 °C, dry, protected from light
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Labeling Capacity: Up to 15 nmol protein per 50 nmol dye vial
Applications
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Fluorescent protein labeling and antibody conjugation
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Low-background imaging in complex biological samples
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Multiplex assays and biomarker detection
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Near-infrared immunoassays and flow cytometry
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High-sensitivity diagnostic and detection platforms
Why Choose TurboTag™ 652?
TurboTag 652 delivers efficient, covalent labeling of proteins with the near-infrared fluorophore DY-652, supporting advanced analytical and diagnostic workflows. The dye’s high extinction coefficient and NIR spectral profile minimize background interference and maximize detection sensitivity. With NHS ester chemistry for robust conjugate formation and ready-to-use protocols, TurboTag 652 offers researchers a fast, scalable, and reliable solution for high-performance protein labeling.
