Description
TurboTag 549 Protein Labeling Kit for High-Performance DY-549P1 Conjugation
TurboTag 549 from emp BIOTECH is a complete fluorescent labeling and purification kit designed for covalent conjugation of proteins with DYOMICS’ DY-549P1 fluorophore. Ideal for proteins ≥ 25 kDa—including antibodies—this kit uses a highly reactive NHS ester to form stable amide bonds, enabling efficient fluorescent labeling under mild conditions. The resulting protein-dye conjugates exhibit excitation and emission maxima at 556 nm and 568 nm, respectively, providing a bright and reliable signal for a wide range of biochemical and diagnostic applications.
Each vial delivers 50 nmol of reactive TurboTag 549 dye, suitable for labeling up to 15 nmol of protein. Pre-validated protocols for 1 mg and 100 µg of IgG streamline your workflow, making the kit especially convenient for immunoassay development, fluorescence microscopy, and molecular detection.
Performance Highlights
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Efficient covalent labeling of proteins ≥ 25 kDa, especially antibodies
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Strong fluorescent signal with DY-549P1 (excitation/emission at 556/568 nm)
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NHS ester chemistry ensures stable and reproducible amide linkage formation
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Pre-optimized protocols for labeling 1 mg or 100 µg of IgG
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Kit includes all necessary reagents for labeling and purification
Properties
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Molecular Weight: 1040.06
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Empirical Formula: C₄₀H₄₈N₃O₁₇S₄Na₃
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Absorption [λmax]: 560 ± 3 nm
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Emission [λmax]: 575 ± 3 nm
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Extinction Coefficient (ε): 150,000 M⁻¹·cm⁻¹
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Reactive Moiety: NHS ester (reacts with primary amines to form stable amide linkage)
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Storage: −20 °C, dry, protected from light
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Labeling Capacity: Up to 15 nmol protein per 50 nmol dye vial
Applications
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Fluorescent labeling of proteins and antibodies
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Immunofluorescence and flow cytometry
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High-sensitivity imaging and molecular tracking
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Bioconjugation for diagnostic and therapeutic R&D
Why Choose TurboTag™ 549?
TurboTag 549 enables fast and reproducible fluorescent labeling of high molecular weight proteins using DY-549P1 dye. Its superior extinction coefficient and narrow emission profile make it an excellent choice for fluorescence-based applications requiring high brightness and stability. Designed for ease of use, the kit supports robust labeling with minimal hands-on time, making it ideal for routine or high-throughput protein conjugation workflows.
