Description
TurboTag 648 Protein Labeling Kit for DY-648 Conjugation in Near-Infrared Applications
TurboTag 648 from emp BIOTECH is a high-performance labeling and purification kit for covalent conjugation of proteins using DY-648, a near-infrared fluorophore. Optimized for proteins ≥ 25 kDa—particularly antibodies—the kit employs a highly reactive NHS ester of DY-648 to form stable amide linkages with primary amines, enabling efficient and reproducible protein labeling. The resulting conjugates exhibit strong fluorescence with excitation/emission maxima at 653 nm and 672 nm, ideal for sensitive detection in imaging, diagnostics, and multiplex assays.
Each kit vial provides 50 nmol of reactive dye, sufficient for labeling up to 15 nmol of protein. Pre-optimized protocols for both 1 mg and 100 µg of IgG streamline setup, making TurboTag 648 a powerful solution for reliable, high-sensitivity fluorescence workflows.
Performance Highlights
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Robust fluorescent labeling of proteins ≥ 25 kDa, including IgG and other antibodies
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Near-infrared fluorophore with excitation/emission at 653/672 nm
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Stable covalent conjugation via NHS ester chemistry
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High extinction coefficient (250,000 M⁻¹·cm⁻¹) ensures strong signal output
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Fast and reproducible protocols for small- and medium-scale antibody labeling
Properties
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Molecular Weight: 936
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Empirical Formula: C₄₀H₄₇N₃O₁₄S₃Na₂
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Absorption [λmax]: 653 ± 3 nm
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Emission [λmax]: 672 ± 3 nm
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Extinction Coefficient (ε): 250,000 M⁻¹·cm⁻¹
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Reactive Moiety: NHS ester (reacts with primary amines to form stable amide linkage)
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Storage: −20 °C, dry, protected from light
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Labeling Capacity: Up to 15 nmol protein per 50 nmol dye vial
Applications
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Antibody and protein labeling for near-infrared fluorescence detection
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High-sensitivity multiplex immunoassays
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Low-background fluorescence imaging
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Diagnostic and analytical applications requiring NIR dyes
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Signal amplification in biomarker research
Why Choose TurboTag™ 648?
TurboTag 648 offers precise, high-efficiency protein labeling with DY-648, delivering powerful near-infrared fluorescence for complex biological assays. Its superior photophysical properties—combined with stable NHS ester chemistry—enable reproducible conjugation under mild conditions. Whether for in vitro diagnostics, immunoassays, or imaging platforms, TurboTag 648 ensures outstanding performance, scalability, and ease of use in advanced protein detection workflows.
